Detailed information of TA system TA123543

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 1018368..1018954
• Replicon: chromosome
• Accession: NZ_CP039127
• Organism: Pseudomonas sp. A2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E5221_RS04785
• Protein ID: WP_080604915.1
• Coordinates: 1018368..1018661 (+)
• Length: 98 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: A0A0C1KRT9
• Locus tag: E5221_RS04790
• Protein ID: WP_039613839.1
• Coordinates: 1018658..1018954 (+)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5221_RS04760 (E5221_04900)	1013879..1014733	+	855	WP_013970992.1	putative selenate ABC transporter substrate-binding protein	-
E5221_RS04765 (E5221_04905)	1014754..1015548	+	795	WP_061552022.1	phosphonate ABC transporter ATP-binding protein	-
E5221_RS04770 (E5221_04910)	1015542..1016369	+	828	WP_061551916.1	ABC transporter permease	-
E5221_RS04775 (E5221_04915)	1016366..1017133	+	768	WP_061551917.1	phosphonate ABC transporter, permease protein PhnE	-
E5221_RS04780 (E5221_04920)	1017260..1018147	-	888	WP_061551918.1	bestrophin family ion channel	-
E5221_RS04785 (E5221_04925)	1018368..1018661	+	294	WP_080604915.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5221_RS04790 (E5221_04930)	1018658..1018954	+	297	WP_039613839.1	putative addiction module antidote protein	Antitoxin
E5221_RS04795 (E5221_04935)	1019108..1020005	-	898	Protein_924	transporter substrate-binding domain-containing protein	-
E5221_RS04800 (E5221_04940)	1020032..1020382	-	351	WP_013971005.1	RidA family protein	-
E5221_RS04805 (E5221_04945)	1020410..1021651	-	1242	WP_182329839.1	D-amino acid dehydrogenase	-
E5221_RS04810 (E5221_04950)	1021759..1022664	+	906	WP_013971007.1	LysR substrate-binding domain-containing protein	-
E5221_RS04815 (E5221_04955)	1022833..1023066	-	234	WP_247846922.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (22-86)

Antitoxin

• Uncharacterized protein HI1420 (5-91)

Sequences

Toxin

Protein

• Length: 98 a.a.
• Molecular weight: 10793.69 Da
• Isoelectric Point: 10.5815

>T123543 WP_080604915.1 NZ_CP039127:1018368-1018661 [Pseudomonas sp. A2]
MYLVEQTESFAVWLSSLRDLRAQLAIGRRLERAAMGNLGDVKWLGGGIGELRIDVAAGYRVYFAQKGQRLVLLLVGGDKS
TQATDILKARKLLKEMK

Nucleotide

• Length: 294 bp

>T123543 NZ_CP039127:1018368-1018661 [Pseudomonas sp. A2]
ATGTATCTCGTTGAACAGACGGAGAGCTTTGCAGTTTGGCTATCCAGCTTGAGAGATTTGAGGGCCCAGCTTGCGATAGG
TCGTAGATTGGAGCGAGCAGCAATGGGGAATCTTGGAGATGTCAAATGGCTTGGAGGCGGTATCGGTGAGCTTCGCATCG
ATGTAGCTGCTGGATATCGTGTCTATTTTGCGCAAAAGGGCCAGAGGCTGGTTCTGCTGTTGGTCGGCGGAGACAAGTCC
ACCCAGGCCACCGATATCCTCAAAGCCAGAAAACTGCTGAAGGAGATGAAATGA

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 10630.32 Da
• Isoelectric Point: 5.6864

>AT123543 WP_039613839.1 NZ_CP039127:1018658-1018954 [Pseudomonas sp. A2]
MSEKLIPFDMAALLSSDEAITEYLSQVLADGDTEEIIRALGHIARARGMTQIATESGLGRESLYKALSPGAKPRFDTVLK
VIRALGVDLLVQPHVVPR

Nucleotide

• Length: 297 bp

>AT123543 NZ_CP039127:1018658-1018954 [Pseudomonas sp. A2]
ATGAGCGAAAAGTTGATCCCGTTTGATATGGCGGCCTTGTTGAGTAGTGATGAGGCCATCACTGAGTACCTTAGTCAGGT
CCTCGCAGATGGCGATACCGAGGAAATAATTCGGGCACTTGGCCATATCGCTCGCGCACGGGGAATGACGCAGATCGCCA
CCGAGAGTGGGTTGGGAAGGGAAAGTCTGTACAAGGCACTCAGTCCAGGCGCAAAACCACGCTTTGACACTGTGCTGAAA
GTCATTCGTGCCTTGGGGGTCGACCTCCTCGTTCAGCCCCATGTAGTTCCCCGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A0C1KRT9