Detailed information of TA system TA123542

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/upstrm_HI1419-dnstrm_HI1420
• Location: 386271..386873
• Replicon: chromosome
• Accession: NZ_CP039127
• Organism: Pseudomonas sp. A2

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E5221_RS01835
• Protein ID: WP_061550702.1
• Coordinates: 386271..386561 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A2N5BJ40
• Locus tag: E5221_RS01840
• Protein ID: WP_015268632.1
• Coordinates: 386571..386873 (+)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5221_RS01815 (E5221_01840)	381639..382412	-	774	WP_003257472.1	ABC transporter ATP-binding protein	-
E5221_RS01820 (E5221_01845)	383078..384463	+	1386	WP_025337364.1	GABA permease	-
E5221_RS01825 (E5221_01850)	384638..385081	-	444	WP_013970488.1	hypothetical protein	-
E5221_RS01830 (E5221_01855)	385141..386121	-	981	WP_061550701.1	polyphosphate kinase 2	-
E5221_RS01835 (E5221_01860)	386271..386561	+	291	WP_061550702.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E5221_RS01840 (E5221_01865)	386571..386873	+	303	WP_015268632.1	putative addiction module antidote protein	Antitoxin
E5221_RS01850 (E5221_01875)	387178..387450	-	273	WP_003257478.1	oxidative damage protection protein	-
E5221_RS01855 (E5221_01880)	387447..388514	-	1068	WP_182329366.1	A/G-specific adenine glycosylase	-
E5221_RS01860 (E5221_01885)	388511..390757	-	2247	WP_232880988.1	AsmA family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	348415..393648	45233

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-96)

Antitoxin

• Cro/C1-type helix-turn-helix domain (46-90)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 10982.83 Da
• Isoelectric Point: 10.7785

>T123542 WP_061550702.1 NZ_CP039127:386271-386561 [Pseudomonas sp. A2]
MRTLIQTETFRRWFAALRDPRAKARINVRLRRVELGVMGDCKPVAEGVSELRIDYGPGYRVYFVQRGYEVIILLAGGDKT
SQARDIKSALKLARDL

Nucleotide

• Length: 291 bp

>T123542 NZ_CP039127:386271-386561 [Pseudomonas sp. A2]
ATGCGCACACTAATTCAAACCGAAACCTTCCGAAGGTGGTTCGCTGCTTTACGGGATCCGCGGGCAAAGGCCCGGATAAA
CGTCAGGCTGCGTCGAGTGGAGCTTGGAGTGATGGGGGACTGTAAACCGGTTGCCGAGGGAGTATCCGAGCTTCGGATCG
ACTATGGTCCGGGTTATCGGGTGTATTTTGTTCAACGAGGCTATGAAGTGATCATCCTCCTGGCGGGTGGCGATAAAACC
AGCCAGGCCAGAGATATCAAATCTGCCCTCAAGCTGGCACGCGACCTTTAG

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 10704.41 Da
• Isoelectric Point: 5.1131

>AT123542 WP_015268632.1 NZ_CP039127:386571-386873 [Pseudomonas sp. A2]
MTNIKLTKWDAVDHLKTEEDMALYLEACLEENDPALLAAALGDIARARGMAQLARDTGLTREGLYKALSAEGNPSLATIM
KVMAALGVRLHAEVLPRAAG

Nucleotide

• Length: 303 bp

>AT123542 NZ_CP039127:386571-386873 [Pseudomonas sp. A2]
ATGACAAATATCAAGCTGACCAAATGGGACGCTGTAGACCATTTGAAGACAGAGGAAGACATGGCCCTCTACCTTGAGGC
CTGTCTTGAAGAAAACGATCCAGCACTGCTTGCTGCTGCCCTGGGTGATATCGCTCGTGCCCGGGGTATGGCTCAGTTGG
CGCGTGATACCGGCCTGACACGAGAAGGGTTGTATAAGGCGTTGTCTGCAGAGGGTAACCCGAGTTTGGCGACCATCATG
AAAGTGATGGCGGCCCTCGGGGTCAGGCTTCATGCCGAGGTGCTGCCGCGCGCGGCGGGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2N5BJ40