Detailed information of TA system TA123541

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relE-higA/HigB-HigA
• Location: 343322..344195
• Replicon: chromosome
• Accession: NZ_CP039127
• Organism: Pseudomonas sp. A2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: E5221_RS01565
• Protein ID: WP_102083354.1
• Coordinates: 343322..343675 (+)
• Length: 118 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E5221_RS01570
• Protein ID: WP_061550697.1
• Coordinates: 343722..344195 (+)
• Length: 158 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5221_RS01550 (E5221_01585)	339338..340057	-	720	WP_015268622.1	response regulator	-
E5221_RS01555 (E5221_01590)	340198..342495	+	2298	WP_247846792.1	TonB-dependent siderophore receptor	-
E5221_RS01560 (E5221_01595)	342938..343144	-	207	WP_082810501.1	DUF3079 domain-containing protein	-
E5221_RS01565 (E5221_01600)	343322..343675	+	354	WP_102083354.1	type II toxin-antitoxin system HigB family toxin	Toxin
E5221_RS01570 (E5221_01605)	343722..344195	+	474	WP_061550697.1	transcriptional regulator	Antitoxin
E5221_RS01575 (E5221_01610)	344719..345702	+	984	WP_122048507.1	hypothetical protein	-
E5221_RS01580 (E5221_01615)	345725..346690	-	966	WP_163002516.1	SGNH/GDSL hydrolase family protein	-
E5221_RS01585 (E5221_01620)	346687..348414	-	1728	WP_122048505.1	SGNH/GDSL hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (73-111)

Antitoxin

• Antitoxin HigA (43-154)

Sequences

Toxin

Protein

• Length: 118 a.a.
• Molecular weight: 13588.73 Da
• Isoelectric Point: 10.4174

>T123541 WP_102083354.1 NZ_CP039127:343322-343675 [Pseudomonas sp. A2]
MRVITKAAVTKAIEAHAQWKAPLSLWLTTFDRATLRFESFEQLRNTWSTASGWNVDRIPCSKLRPSSRKGPLDVYVFDIK
KNECRLIAWLNTRTGTLYIKAILSHAEYDKWCKSDVR

Nucleotide

• Length: 354 bp

>T123541 NZ_CP039127:343322-343675 [Pseudomonas sp. A2]
ATGCGGGTGATAACGAAAGCGGCGGTTACCAAAGCCATCGAAGCTCACGCCCAGTGGAAAGCCCCCCTGAGCCTGTGGCT
CACGACCTTTGATAGGGCGACGCTGCGCTTCGAGTCTTTTGAGCAGCTAAGGAATACGTGGTCTACCGCCTCTGGCTGGA
ACGTTGACCGCATTCCTTGTTCAAAACTCAGACCCTCCAGCCGAAAAGGGCCGCTTGATGTCTATGTGTTCGATATCAAG
AAAAACGAATGCCGTCTGATCGCATGGCTCAACACACGAACGGGCACCCTCTATATCAAGGCCATTCTGTCGCACGCGGA
GTACGACAAGTGGTGCAAGAGCGATGTCAGGTAG

Antitoxin

Protein

• Length: 158 a.a.
• Molecular weight: 17198.66 Da
• Isoelectric Point: 4.5725

>AT123541 WP_061550697.1 NZ_CP039127:343722-344195 [Pseudomonas sp. A2]
MKRDKIEANGLDAFIANISPMLDGLNAQMATMAQLLAACHDPIKDDAELQARMALIDELYSHADSEGHAAARFAEMVADR
VYEYESESVLVPFASQAEALAFLLSDRGVKQKDLASIATQSAVSEILNNKRKMTVAQIKGFAEFFSVPVEFFMHGVV

Nucleotide

• Length: 474 bp

>AT123541 NZ_CP039127:343722-344195 [Pseudomonas sp. A2]
ATGAAGCGGGATAAAATCGAAGCCAATGGACTAGATGCCTTCATCGCTAATATTTCACCCATGCTGGATGGCCTGAACGC
CCAGATGGCAACCATGGCCCAGCTTCTTGCTGCCTGCCATGACCCTATCAAGGACGATGCAGAGCTCCAGGCGCGGATGG
CATTGATCGATGAACTCTACTCGCATGCCGACAGCGAAGGTCACGCAGCAGCGCGCTTTGCGGAGATGGTGGCGGATCGT
GTCTACGAGTATGAGAGTGAGTCGGTACTCGTGCCGTTTGCGAGCCAGGCCGAAGCGTTGGCCTTCCTGCTGTCGGACAG
GGGCGTGAAGCAGAAGGACCTTGCCTCGATAGCGACCCAGAGTGCGGTTTCAGAGATTCTGAACAACAAGCGGAAGATGA
CGGTTGCCCAGATAAAGGGCTTTGCTGAGTTTTTCAGCGTGCCGGTCGAGTTTTTCATGCATGGGGTGGTTTGA