Detailed information of TA system TA123534

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2672952..2673715
• Replicon: chromosome
• Accession: NZ_CP039112
• Organism: Piscirickettsia salmonis strain Psal-135

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Psal135_RS13535
• Protein ID: WP_144420759.1
• Coordinates: 2672952..2673452 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: Psal135_RS13540
• Protein ID: WP_017376501.1
• Coordinates: 2673449..2673715 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal135_RS13505	2667964..2669079	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
Psal135_RS13510	2669242..2669766	+	525	WP_017376000.1	inorganic diphosphatase	-
Psal135_RS13515	2669766..2670296	+	531	WP_017375999.1	transcriptional repressor	-
Psal135_RS13520	2670627..2670866	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
Psal135_RS18595	2670791..2671453	+	663	WP_174872532.1	transposase	-
Psal135_RS18600	2671457..2671762	+	306	WP_146619445.1	transposase	-
Psal135_RS13530	2671956..2672831	+	876	WP_048875904.1	IS982 family transposase	-
Psal135_RS13535	2672952..2673452	-	501	WP_144420759.1	GNAT family N-acetyltransferase	Toxin
Psal135_RS13540	2673449..2673715	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
Psal135_RS13545	2673881..2674855	-	975	WP_048875903.1	IS30 family transposase	-
Psal135_RS13550	2675035..2675655	-	621	WP_075275269.1	transposase	-
Psal135_RS13555	2675652..2675948	-	297	Protein_2664	transposase	-
Psal135_RS13560	2675962..2677365	-	1404	WP_048876031.1	IS4 family transposase	-
Psal135_RS13565	2677489..2678076	-	588	Protein_2666	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2671956..2678076	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18558.59 Da
• Isoelectric Point: 9.0858

>T123534 WP_144420759.1 NZ_CP039112:c2673452-2672952 [Piscirickettsia salmonis]
ITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPETIP
VIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKIRQL
LKNETK

Nucleotide

• Length: 501 bp

>T123534 NZ_CP039112:c2673452-2672952 [Piscirickettsia salmonis]
ATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAATGAGTGGTT
ACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAGTGATTGGCT
ATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAGACAATTCCG
GTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAAAGATGCTTT
AATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAGCAAAACAGT
TTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATTAGGCAACTT
CTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT123534 WP_017376501.1 NZ_CP039112:c2673715-2673449 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT123534 NZ_CP039112:c2673715-2673449 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L6TGZ1