Detailed information of TA system TA123528

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2672925..2673688
• Replicon: chromosome
• Accession: NZ_CP039107
• Organism: Piscirickettsia salmonis strain Psal-134

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Psal134_RS13540
• Protein ID: WP_144420759.1
• Coordinates: 2672925..2673425 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: Psal134_RS13545
• Protein ID: WP_017376501.1
• Coordinates: 2673422..2673688 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal134_RS13510	2667937..2669052	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
Psal134_RS13515	2669215..2669739	+	525	WP_017376000.1	inorganic diphosphatase	-
Psal134_RS13520	2669739..2670269	+	531	WP_017375999.1	transcriptional repressor	-
Psal134_RS13525	2670600..2670839	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
Psal134_RS18670	2670764..2671426	+	663	WP_174872532.1	transposase	-
Psal134_RS18675	2671430..2671735	+	306	WP_146619445.1	transposase	-
Psal134_RS13535	2671929..2672804	+	876	WP_048875904.1	IS982 family transposase	-
Psal134_RS13540	2672925..2673425	-	501	WP_144420759.1	GNAT family N-acetyltransferase	Toxin
Psal134_RS13545	2673422..2673688	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
Psal134_RS13550	2673854..2674828	-	975	WP_048875903.1	IS30 family transposase	-
Psal134_RS13555	2675008..2675628	-	621	WP_075275269.1	transposase	-
Psal134_RS13560	2675625..2675921	-	297	Protein_2673	transposase	-
Psal134_RS13565	2675935..2677338	-	1404	WP_048876031.1	IS4 family transposase	-
Psal134_RS13570	2677462..2678049	-	588	Protein_2675	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2671929..2678049	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18558.59 Da
• Isoelectric Point: 9.0858

>T123528 WP_144420759.1 NZ_CP039107:c2673425-2672925 [Piscirickettsia salmonis]
ITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPETIP
VIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKIRQL
LKNETK

Nucleotide

• Length: 501 bp

>T123528 NZ_CP039107:c2673425-2672925 [Piscirickettsia salmonis]
ATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAATGAGTGGTT
ACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAGTGATTGGCT
ATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAGACAATTCCG
GTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAAAGATGCTTT
AATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAGCAAAACAGT
TTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATTAGGCAACTT
CTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT123528 WP_017376501.1 NZ_CP039107:c2673688-2673422 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT123528 NZ_CP039107:c2673688-2673422 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L6TGZ1