Detailed information of TA system TA123527

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 5705..6311
• Replicon: plasmid unnamed4
• Accession: NZ_CP039106
• Organism: Piscirickettsia salmonis strain Psal-118

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: Psal118_RS17910
• Protein ID: WP_027242955.1
• Coordinates: 5705..5965 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: Psal118_RS17915
• Protein ID: WP_016211078.1
• Coordinates: 5958..6311 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal118_RS17880	994..1977	+	984	WP_016211499.1	ParB/RepB/Spo0J family partition protein	-
Psal118_RS18735	2454..2729	+	276	WP_171824216.1	hypothetical protein	-
Psal118_RS17890	2934..3377	+	444	WP_178099494.1	transposase	-
Psal118_RS17895	3374..4348	-	975	WP_036771330.1	IS30 family transposase	-
Psal118_RS18740	4462..4818	+	357	WP_075278739.1	transposase	-
Psal118_RS17905	4884..5474	-	591	WP_016212329.1	site-specific integrase	-
Psal118_RS17910	5705..5965	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
Psal118_RS17915	5958..6311	+	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Psal118_RS17920	6488..7462	+	975	WP_069971681.1	IS30 family transposase	-
Psal118_RS17925	7571..7798	+	228	Protein_10	hypothetical protein	-
Psal118_RS17930	7995..8360	+	366	WP_027242954.1	hypothetical protein	-
Psal118_RS17935	8505..8759	+	255	WP_027242953.1	type II toxin-antitoxin system HicA family toxin	-
Psal118_RS17940	8752..9099	+	348	WP_027242952.1	type II toxin-antitoxin system HicB family antitoxin	-
Psal118_RS17945	9197..10171	+	975	WP_036771330.1	IS30 family transposase	-
Psal118_RS18745	10403..10543	-	141	WP_162039123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33526	33526

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T123527 WP_027242955.1 NZ_CP039106:5705-5965 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T123527 NZ_CP039106:5705-5965 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT123527 WP_016211078.1 NZ_CP039106:5958-6311 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT123527 NZ_CP039106:5958-6311 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87

Antitoxin

• AlphaFold DB: A0A7U4WZG4