Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 48130..48773 Replicon plasmid unnamed1
Accession NZ_CP039093
Organism Piscirickettsia salmonis strain Psal-114

Toxin (Protein)


Gene name vapC Uniprot ID A0A1L6TI66
Locus tag Psal114_RS16505 Protein ID WP_027242931.1
Coordinates 48366..48773 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A1L6TI10
Locus tag Psal114_RS16500 Protein ID WP_027242932.1
Coordinates 48130..48357 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Psal114_RS16470 43387..44430 - 1044 WP_017375960.1 NAD(P)-dependent alcohol dehydrogenase -
Psal114_RS16475 44732..44965 + 234 WP_017375959.1 type II toxin-antitoxin system RelE/ParE family toxin -
Psal114_RS16480 45103..45252 - 150 WP_196214251.1 hypothetical protein -
Psal114_RS16485 45318..45647 + 330 WP_162038692.1 HigA family addiction module antidote protein -
Psal114_RS16490 46089..47063 + 975 WP_036773116.1 IS30 family transposase -
Psal114_RS16495 47632..47997 + 366 WP_026063496.1 hypothetical protein -
Psal114_RS16500 48130..48357 + 228 WP_027242932.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
Psal114_RS16505 48366..48773 + 408 WP_027242931.1 DUF4411 family protein Toxin
Psal114_RS16510 48919..50301 + 1383 WP_027242930.1 hypothetical protein -
Psal114_RS16515 50491..50694 + 204 WP_017375952.1 hypothetical protein -
Psal114_RS16520 50890..51273 + 384 WP_027242929.1 HNH endonuclease -
Psal114_RS16525 51360..51842 + 483 WP_075278733.1 P27 family phage terminase small subunit -
Psal114_RS16530 51845..53176 + 1332 WP_048876205.1 terminase large subunit -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..176237 176237


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-127)

Antitoxin

SpoVT-AbrB domain

(7-41)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 15562.93 Da        Isoelectric Point: 7.4168

>T123512 WP_027242931.1 NZ_CP039093:48366-48773 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLDVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVDHLELEDWAS

Download         Length: 408 bp

>T123512 NZ_CP039093:48366-48773 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAACCTCGCTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCGATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGCAGTCAAC
ACCAGCGAAAAAATCAGGAAGCAATTAATACCTTGGTGAATAAGCTCGATGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCACATGC
GCTTTCAGAAAAGGCTGTTTTAGTAACGCATAATACAAAAGAGTTTCAGCGTGTCGATCATTTAGAGCTTGAAGACTGGG
CAAGTTAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8948.07 Da        Isoelectric Point: 4.5700

>AT123512 WP_027242932.1 NZ_CP039093:48130-48357 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDRQDIQAQERDWF

Download         Length: 228 bp

>AT123512 NZ_CP039093:48130-48357 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCTCAAGCCGTTCGATTACCGAAAGAAATGCGCCTTGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAGGATCGCCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6TI66

Antitoxin

Source ID Structure
AlphaFold DB A0A1L6TI10

References