Detailed information of TA system TA123497

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 5734..6340
• Replicon: plasmid unnamed4
• Accession: NZ_CP039080
• Organism: Piscirickettsia salmonis strain Psal-109

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: Psal109_RS17960
• Protein ID: WP_027242955.1
• Coordinates: 5734..5994 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: Psal109_RS17965
• Protein ID: WP_016211078.1
• Coordinates: 5987..6340 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal109_RS17930	994..1977	+	984	WP_016211499.1	ParB/RepB/Spo0J family partition protein	-
Psal109_RS18720	2483..2758	+	276	WP_171824216.1	hypothetical protein	-
Psal109_RS17940	2963..3406	+	444	WP_178099494.1	transposase	-
Psal109_RS17945	3403..4377	-	975	WP_036771330.1	IS30 family transposase	-
Psal109_RS18725	4491..4847	+	357	WP_075278739.1	transposase	-
Psal109_RS17955	4913..5503	-	591	WP_016212329.1	site-specific integrase	-
Psal109_RS17960	5734..5994	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
Psal109_RS17965	5987..6340	+	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Psal109_RS17970	6517..7491	+	975	WP_069971681.1	IS30 family transposase	-
Psal109_RS17975	7600..7827	+	228	Protein_10	hypothetical protein	-
Psal109_RS17980	8024..8389	+	366	WP_027242954.1	hypothetical protein	-
Psal109_RS17985	8534..8788	+	255	WP_027242953.1	type II toxin-antitoxin system HicA family toxin	-
Psal109_RS17990	8781..9128	+	348	WP_027242952.1	type II toxin-antitoxin system HicB family antitoxin	-
Psal109_RS17995	9226..10200	+	975	WP_036771330.1	IS30 family transposase	-
Psal109_RS18730	10432..10572	-	141	WP_162039123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33555	33555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T123497 WP_027242955.1 NZ_CP039080:5734-5994 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T123497 NZ_CP039080:5734-5994 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT123497 WP_016211078.1 NZ_CP039080:5987-6340 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT123497 NZ_CP039080:5987-6340 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87

Antitoxin

• AlphaFold DB: A0A7U4WZG4