Detailed information of TA system TA123491

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 5572..6178
• Replicon: plasmid unnamed4
• Accession: NZ_CP039074
• Organism: Piscirickettsia salmonis strain Psal-108

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: Psal108_RS18045
• Protein ID: WP_027242955.1
• Coordinates: 5572..5832 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: Psal108_RS18050
• Protein ID: WP_016211078.1
• Coordinates: 5825..6178 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal108_RS18015	832..1815	+	984	WP_016211499.1	ParB/RepB/Spo0J family partition protein	-
Psal108_RS18910	2321..2596	+	276	WP_171824216.1	hypothetical protein	-
Psal108_RS18025	2801..3244	+	444	WP_178099494.1	transposase	-
Psal108_RS18030	3241..4215	-	975	WP_036771330.1	IS30 family transposase	-
Psal108_RS18915	4329..4685	+	357	WP_075278739.1	transposase	-
Psal108_RS18040	4751..5341	-	591	WP_016212329.1	site-specific integrase	-
Psal108_RS18045	5572..5832	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
Psal108_RS18050	5825..6178	+	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Psal108_RS18055	6355..7329	+	975	WP_069971681.1	IS30 family transposase	-
Psal108_RS18060	7438..7665	+	228	Protein_10	hypothetical protein	-
Psal108_RS18065	7862..8227	+	366	WP_027242954.1	hypothetical protein	-
Psal108_RS18070	8372..8626	+	255	WP_027242953.1	type II toxin-antitoxin system HicA family toxin	-
Psal108_RS18075	8619..8966	+	348	WP_027242952.1	type II toxin-antitoxin system HicB family antitoxin	-
Psal108_RS18080	9064..10038	+	975	WP_036771330.1	IS30 family transposase	-
Psal108_RS18920	10270..10410	-	141	WP_162039123.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33555	33555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T123491 WP_027242955.1 NZ_CP039074:5572-5832 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T123491 NZ_CP039074:5572-5832 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT123491 WP_016211078.1 NZ_CP039074:5825-6178 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT123491 NZ_CP039074:5825-6178 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87

Antitoxin

• AlphaFold DB: A0A7U4WZG4