Detailed information of TA system TA123486

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2672672..2673435
• Replicon: chromosome
• Accession: NZ_CP039070
• Organism: Piscirickettsia salmonis strain Psal-108

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Psal108_RS13575
• Protein ID: WP_144420759.1
• Coordinates: 2672672..2673172 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: Psal108_RS13580
• Protein ID: WP_017376501.1
• Coordinates: 2673169..2673435 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal108_RS13545	2667684..2668799	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
Psal108_RS13550	2668962..2669486	+	525	WP_017376000.1	inorganic diphosphatase	-
Psal108_RS13555	2669486..2670016	+	531	WP_017375999.1	transcriptional repressor	-
Psal108_RS13560	2670347..2670586	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
Psal108_RS18785	2670511..2671173	+	663	WP_174872532.1	transposase	-
Psal108_RS18790	2671177..2671482	+	306	WP_146619445.1	transposase	-
Psal108_RS13570	2671676..2672551	+	876	WP_048875904.1	IS982 family transposase	-
Psal108_RS13575	2672672..2673172	-	501	WP_144420759.1	GNAT family N-acetyltransferase	Toxin
Psal108_RS13580	2673169..2673435	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
Psal108_RS13585	2673601..2674575	-	975	WP_048875903.1	IS30 family transposase	-
Psal108_RS13590	2674755..2675375	-	621	WP_075275269.1	transposase	-
Psal108_RS13595	2675372..2675668	-	297	Protein_2675	transposase	-
Psal108_RS13600	2675682..2677085	-	1404	WP_048876031.1	IS4 family transposase	-
Psal108_RS13605	2677209..2677796	-	588	Protein_2677	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2671676..2677796	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18558.59 Da
• Isoelectric Point: 9.0858

>T123486 WP_144420759.1 NZ_CP039070:c2673172-2672672 [Piscirickettsia salmonis]
ITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPETIP
VIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKIRQL
LKNETK

Nucleotide

• Length: 501 bp

>T123486 NZ_CP039070:c2673172-2672672 [Piscirickettsia salmonis]
ATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAATGAGTGGTT
ACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAGTGATTGGCT
ATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAGACAATTCCG
GTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAAAGATGCTTT
AATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAGCAAAACAGT
TTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATTAGGCAACTT
CTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT123486 WP_017376501.1 NZ_CP039070:c2673435-2673169 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT123486 NZ_CP039070:c2673435-2673169 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L6TGZ1