Detailed information of TA system TA123478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 108544..109187
• Replicon: plasmid unnamed1
• Accession: NZ_CP039061
• Organism: Piscirickettsia salmonis strain Psal-099

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1L6TI66
• Locus tag: Psal099_RS16885
• Protein ID: WP_027242931.1
• Coordinates: 108780..109187 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1L6TI10
• Locus tag: Psal099_RS16880
• Protein ID: WP_027242932.1
• Coordinates: 108544..108771 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal099_RS16845	104062..105039	+	978	WP_036771347.1	IS5 family transposase	-
Psal099_RS16850	105066..105527	+	462	Protein_134	tail assembly protein	-
Psal099_RS16855	105534..105821	-	288	WP_027243212.1	type II toxin-antitoxin system RelE/ParE family toxin	-
Psal099_RS16860	105811..106065	-	255	WP_027243211.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
Psal099_RS16865	106283..106444	-	162	WP_155046636.1	hypothetical protein	-
Psal099_RS16870	106459..107436	-	978	WP_036771347.1	IS5 family transposase	-
Psal099_RS16875	108046..108411	+	366	WP_026063496.1	hypothetical protein	-
Psal099_RS16880	108544..108771	+	228	WP_027242932.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal099_RS16885	108780..109187	+	408	WP_027242931.1	DUF4411 family protein	Toxin
Psal099_RS16890	109333..110715	+	1383	WP_027242930.1	hypothetical protein	-
Psal099_RS16895	110905..111108	+	204	WP_017375952.1	hypothetical protein	-
Psal099_RS16900	111304..111687	+	384	WP_027242929.1	HNH endonuclease	-
Psal099_RS16905	111774..112256	+	483	WP_075278733.1	P27 family phage terminase small subunit	-
Psal099_RS16910	112259..113590	+	1332	WP_048876205.1	terminase large subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..175441	175441

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15562.93 Da
• Isoelectric Point: 7.4168

>T123478 WP_027242931.1 NZ_CP039061:108780-109187 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLDVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVDHLELEDWAS

Nucleotide

• Length: 408 bp

>T123478 NZ_CP039061:108780-109187 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAACCTCGCTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCGATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGCAGTCAAC
ACCAGCGAAAAAATCAGGAAGCAATTAATACCTTGGTGAATAAGCTCGATGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCACATGC
GCTTTCAGAAAAGGCTGTTTTAGTAACGCATAATACAAAAGAGTTTCAGCGTGTCGATCATTTAGAGCTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8948.07 Da
• Isoelectric Point: 4.5700

>AT123478 WP_027242932.1 NZ_CP039061:108544-108771 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDRQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123478 NZ_CP039061:108544-108771 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCTCAAGCCGTTCGATTACCGAAAGAAATGCGCCTTGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAGGATCGCCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: A0A1L6TI66

Antitoxin

• AlphaFold DB: A0A1L6TI10