Detailed information of TA system TA123472

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 63400..64043
• Replicon: plasmid unnamed1
• Accession: NZ_CP039056
• Organism: Piscirickettsia salmonis strain Psal-098

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A1L6TI66
• Locus tag: Psal098_RS16595
• Protein ID: WP_027242931.1
• Coordinates: 63400..63807 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1L6TI10
• Locus tag: Psal098_RS16600
• Protein ID: WP_027242932.1
• Coordinates: 63816..64043 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal098_RS16570	58997..60328	-	1332	WP_048876205.1	terminase large subunit	-
Psal098_RS16575	60331..60813	-	483	WP_075278733.1	P27 family phage terminase small subunit	-
Psal098_RS16580	60900..61283	-	384	WP_027242929.1	HNH endonuclease	-
Psal098_RS16585	61479..61682	-	204	WP_017375952.1	hypothetical protein	-
Psal098_RS16590	61872..63254	-	1383	WP_027242930.1	hypothetical protein	-
Psal098_RS16595	63400..63807	-	408	WP_027242931.1	DUF4411 family protein	Toxin
Psal098_RS16600	63816..64043	-	228	WP_027242932.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal098_RS16605	64176..64541	-	366	WP_026063496.1	hypothetical protein	-
Psal098_RS16610	65628..66605	-	978	WP_036771347.1	IS5 family transposase	-
Psal098_RS16615	66801..66989	+	189	Protein_87	hypothetical protein	-
Psal098_RS16620	67086..68063	+	978	WP_036771347.1	IS5 family transposase	-
Psal098_RS16625	68078..68239	+	162	WP_155046636.1	hypothetical protein	-
Psal098_RS16630	68457..68711	+	255	WP_027243211.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
Psal098_RS16635	68701..68988	+	288	WP_027243212.1	type II toxin-antitoxin system RelE/ParE family toxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..175317	175317

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-127)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15562.93 Da
• Isoelectric Point: 7.4168

>T123472 WP_027242931.1 NZ_CP039056:c63807-63400 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLDVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVDHLELEDWAS

Nucleotide

• Length: 408 bp

>T123472 NZ_CP039056:c63807-63400 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAACCTCGCTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCGATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGCAGTCAAC
ACCAGCGAAAAAATCAGGAAGCAATTAATACCTTGGTGAATAAGCTCGATGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCACATGC
GCTTTCAGAAAAGGCTGTTTTAGTAACGCATAATACAAAAGAGTTTCAGCGTGTCGATCATTTAGAGCTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8948.07 Da
• Isoelectric Point: 4.5700

>AT123472 WP_027242932.1 NZ_CP039056:c64043-63816 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDRQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123472 NZ_CP039056:c64043-63816 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCTCAAGCCGTTCGATTACCGAAAGAAATGCGCCTTGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAGGATCGCCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: A0A1L6TI66

Antitoxin

• AlphaFold DB: A0A1L6TI10