Detailed information of TA system TA123460

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2668876..2669639
• Replicon: chromosome
• Accession: NZ_CP039046
• Organism: Piscirickettsia salmonis strain Psal-073

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Psal073_RS13475
• Protein ID: WP_144420759.1
• Coordinates: 2668876..2669376 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: Psal073_RS13480
• Protein ID: WP_017376501.1
• Coordinates: 2669373..2669639 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal073_RS13445	2663888..2665003	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
Psal073_RS13450	2665166..2665690	+	525	WP_017376000.1	inorganic diphosphatase	-
Psal073_RS13455	2665690..2666220	+	531	WP_017375999.1	transcriptional repressor	-
Psal073_RS13460	2666551..2666790	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
Psal073_RS18520	2666715..2667377	+	663	WP_174872532.1	transposase	-
Psal073_RS18525	2667381..2667686	+	306	WP_146619445.1	transposase	-
Psal073_RS13470	2667880..2668755	+	876	WP_036774017.1	IS982 family transposase	-
Psal073_RS13475	2668876..2669376	-	501	WP_144420759.1	GNAT family N-acetyltransferase	Toxin
Psal073_RS13480	2669373..2669639	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
Psal073_RS13485	2669805..2670779	-	975	WP_048875903.1	IS30 family transposase	-
Psal073_RS13490	2670959..2671579	-	621	WP_075275269.1	transposase	-
Psal073_RS13495	2671576..2671872	-	297	Protein_2643	transposase	-
Psal073_RS13500	2671886..2673289	-	1404	WP_053856766.1	IS4 family transposase	-
Psal073_RS13505	2673413..2674000	-	588	Protein_2645	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2667880..2674000	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18558.59 Da
• Isoelectric Point: 9.0858

>T123460 WP_144420759.1 NZ_CP039046:c2669376-2668876 [Piscirickettsia salmonis]
ITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPETIP
VIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKIRQL
LKNETK

Nucleotide

• Length: 501 bp

>T123460 NZ_CP039046:c2669376-2668876 [Piscirickettsia salmonis]
ATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAATGAGTGGTT
ACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAGTGATTGGCT
ATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAGACAATTCCG
GTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAAAGATGCTTT
AATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAGCAAAACAGT
TTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATTAGGCAACTT
CTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT123460 WP_017376501.1 NZ_CP039046:c2669639-2669373 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT123460 NZ_CP039046:c2669639-2669373 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L6TGZ1