Detailed information of TA system TA123457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 109430..110000
• Replicon: plasmid unnamed2
• Accession: NZ_CP039042
• Organism: Piscirickettsia salmonis strain Psal-072

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: Psal072_RS18230
• Protein ID: WP_036781349.1
• Coordinates: 109674..110000 (+)
• Length: 109 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: Psal072_RS18225
• Protein ID: WP_016212365.1
• Coordinates: 109430..109672 (+)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal072_RS19840	105271..106002	-	732	WP_016212311.1	hypothetical protein	-
Psal072_RS18210	106123..107706	-	1584	WP_052047129.1	protein kinase	-
Psal072_RS18215	107989..108717	-	729	WP_075274745.1	IS6-like element ISPsa2 family transposase	-
Psal072_RS18220	108785..109225	-	441	Protein_149	tyrosine-type recombinase/integrase	-
Psal072_RS18225	109430..109672	+	243	WP_016212365.1	antidote-toxin recognition MazE family protein	Antitoxin
Psal072_RS18230	109674..110000	+	327	WP_036781349.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
Psal072_RS18235	110116..110844	+	729	WP_054300202.1	IS6 family transposase	-
Psal072_RS18240	111314..111697	+	384	WP_016212152.1	hypothetical protein	-
Psal072_RS18245	112004..112381	-	378	WP_016212154.1	DDE-type integrase/transposase/recombinase	-
Psal072_RS18250	112546..112878	-	333	WP_155047283.1	transposase	-
Psal072_RS18260	113067..113846	-	780	WP_081377351.1	transposase	-
Psal072_RS18265	113947..114204	-	258	Protein_157	DDE-type integrase/transposase/recombinase	-
Psal072_RS19845	114493..114612	-	120	Protein_158	IS6 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..192523	192523
	-	inside	IScluster/Tn	-	-	104383..121658	17275

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-104)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 109 a.a.
• Molecular weight: 11971.89 Da
• Isoelectric Point: 10.1733

>T123457 WP_036781349.1 NZ_CP039042:109674-110000 [Piscirickettsia salmonis]
VPERGDIIFLSLDPTLGSEQRGNRPFLVLTSKPYNLKVGLVTGCPVTSKIKGYPFEVELPKNNSVHGAVLVNQLTTKDFR
ARGWNHADKLDKKSLERVQGLIDVMLKG

Nucleotide

• Length: 327 bp

>T123457 NZ_CP039042:109674-110000 [Piscirickettsia salmonis]
GTGCCTGAACGTGGTGATATTATTTTTCTTAGTTTAGACCCTACATTAGGTAGTGAACAAAGAGGAAACCGACCCTTTTT
AGTGTTGACTTCCAAGCCCTATAACTTAAAGGTGGGACTTGTGACGGGGTGCCCGGTTACTTCAAAGATTAAAGGATATC
CTTTTGAAGTTGAGCTACCTAAAAACAATAGCGTTCACGGAGCAGTGTTGGTTAACCAACTAACAACAAAAGATTTCCGT
GCTAGGGGCTGGAATCATGCTGATAAGCTCGATAAAAAATCTCTTGAAAGAGTTCAGGGGCTAATTGATGTGATGTTGAA
AGGTTAA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8950.09 Da
• Isoelectric Point: 4.4750

>AT123457 WP_016212365.1 NZ_CP039042:109430-109672 [Piscirickettsia salmonis]
MDAVVRKWGNSLGLLLPLSIARELHINDGSHVTIDKVGDEIHIKPLTHTQTLELLLSEITPENSHDLVDWGEELGGERFE

Nucleotide

• Length: 243 bp

>AT123457 NZ_CP039042:109430-109672 [Piscirickettsia salmonis]
ATGGATGCAGTTGTTAGAAAATGGGGGAATAGCTTGGGGTTGTTGCTACCCTTGAGCATCGCACGTGAACTTCATATCAA
TGATGGTAGTCATGTGACGATTGATAAGGTCGGGGATGAGATTCACATTAAACCTTTGACTCATACGCAGACCCTTGAAT
TATTATTATCAGAAATTACCCCTGAAAATAGTCATGATTTAGTAGATTGGGGTGAAGAATTAGGAGGGGAGCGCTTTGAA
TAA