Detailed information of TA system TA123444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 23915..24503
• Replicon: plasmid unnamed2
• Accession: NZ_CP039026
• Organism: Acinetobacter baumannii ATCC 17978 substr. PMR-High

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: E5A72_RS19395
• Protein ID: WP_000438826.1
• Coordinates: 24216..24503 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A858UV00
• Locus tag: E5A72_RS19390
• Protein ID: WP_061855757.1
• Coordinates: 23915..24229 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5A72_RS19370	19891..20262	+	372	WP_000504218.1	hypothetical protein	-
E5A72_RS19375	20262..20459	+	198	WP_001282483.1	hypothetical protein	-
E5A72_RS19380	20581..21279	-	699	WP_000007662.1	CPBP family intramembrane metalloprotease	-
E5A72_RS19385	21376..23786	-	2411	Protein_28	TonB-dependent receptor ZnuD2	-
E5A72_RS19390	23915..24229	-	315	WP_061855757.1	BrnA antitoxin family protein	Antitoxin
E5A72_RS19395	24216..24503	-	288	WP_000438826.1	BrnT family toxin	Toxin
E5A72_RS19400	24850..25621	-	772	Protein_31	glucose 1-dehydrogenase	-
E5A72_RS19405	25719..26919	-	1201	Protein_32	alpha/beta fold hydrolase	-
E5A72_RS19410	27135..27428	-	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E5A72_RS19415	27418..27663	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
E5A72_RS19420	28453..29025	-	573	Protein_35	plasmid replication DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30410	30410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T123444 WP_000438826.1 NZ_CP039026:c24503-24216 [Acinetobacter baumannii ATCC 17978]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T123444 NZ_CP039026:c24503-24216 [Acinetobacter baumannii ATCC 17978]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.88 Da
• Isoelectric Point: 8.4628

>AT123444 WP_061855757.1 NZ_CP039026:c24229-23915 [Acinetobacter baumannii ATCC 17978]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT123444 NZ_CP039026:c24229-23915 [Acinetobacter baumannii ATCC 17978]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTAAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: A0A858UV00