Detailed information of TA system TA123442

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 12615..13203
• Replicon: plasmid unnamed2
• Accession: NZ_CP039026
• Organism: Acinetobacter baumannii ATCC 17978 substr. PMR-High

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: E5A72_RS19320
• Protein ID: WP_000438826.1
• Coordinates: 12916..13203 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: A0A858UV00
• Locus tag: E5A72_RS19315
• Protein ID: WP_061855757.1
• Coordinates: 12615..12929 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5A72_RS19295	8590..8961	+	372	WP_000504218.1	hypothetical protein	-
E5A72_RS19300	8961..9158	+	198	WP_001282483.1	hypothetical protein	-
E5A72_RS19305	9280..9978	-	699	WP_000007662.1	CPBP family intramembrane metalloprotease	-
E5A72_RS19310	10075..12486	-	2412	WP_000932945.1	TonB-dependent receptor ZnuD2	-
E5A72_RS19315	12615..12929	-	315	WP_061855757.1	BrnA antitoxin family protein	Antitoxin
E5A72_RS19320	12916..13203	-	288	WP_000438826.1	BrnT family toxin	Toxin
E5A72_RS19325	13550..14323	-	774	WP_061855758.1	glucose 1-dehydrogenase	-
E5A72_RS19330	14421..15623	-	1203	WP_061855756.1	alpha/beta fold hydrolase	-
E5A72_RS19335	15841..16134	-	294	WP_000221358.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E5A72_RS19340	16124..16369	-	246	WP_000246756.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
E5A72_RS19345	16613..16816	+	204	WP_000482656.1	hypothetical protein	-
E5A72_RS19545	17001..17144	-	144	WP_001125244.1	hypothetical protein	-
E5A72_RS19350	17164..17739	-	576	WP_001096616.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30410	30410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T123442 WP_000438826.1 NZ_CP039026:c13203-12916 [Acinetobacter baumannii ATCC 17978]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T123442 NZ_CP039026:c13203-12916 [Acinetobacter baumannii ATCC 17978]
ATGGAACAGTATTTTGAATGGGATGAGGCTAAGAATCGAAAGAATCAGAAAAAGCATGATATCTCTTTCGAAACTGCAAG
CCTTGTTTTTGAAGATCCATTACGGATCTCAATCCAAGACAGACATACCAATGGTGAAGAACGTTGGCAAACCATTGGAC
GAGTTAAAGGCGTACTAATGTTATTGGTGGCTCACACCATTTTTGATGAAGATGACTGTGAAATCATCCGAATCATTAGT
GCAAGGCAAGTCACTAAAGCGGAGCGAAACAAATATGAGCATGGTTAG

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12227.88 Da
• Isoelectric Point: 8.4628

>AT123442 WP_061855757.1 NZ_CP039026:c12929-12615 [Acinetobacter baumannii ATCC 17978]
MSMVRYSRKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT123442 NZ_CP039026:c12929-12615 [Acinetobacter baumannii ATCC 17978]
ATGAGCATGGTTAGATACTCACGCAAAGAGCTAAATGAGAAATTTGGTGAGAAGCAAGATGCTGAAATTCAACGCTTGCT
TGCTAAAGGCACGGTTCCTGATGATCAGCTAGATCTATCAGATATTCCTGAAATTACAGACTGGAGCAATGCTGTACGCC
AAAATCAATTTTACCGCCCAGTAAAGCAACAAACTTCAATTCGGTTAGATGCTGATGTACTTGCTTGGTTCAAAGCACAA
GGCAAAGGTTATCAAACACGTATGAATGAAATTTTACGAGATGCTATGCTAAAAGAATTAAAAAATCATCAATAA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: A0A858UV00