Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 975141..975748 Replicon chromosome
Accession NZ_CP038996
Organism Enterococcus faecium strain SRR24

Toxin (Protein)


Gene name mazF Uniprot ID E3USU0
Locus tag E6A31_RS04785 Protein ID WP_002321032.1
Coordinates 975398..975748 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A829F3C0
Locus tag E6A31_RS04780 Protein ID WP_002287514.1
Coordinates 975141..975404 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6A31_RS04740 970177..971076 + 900 WP_002322787.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
E6A31_RS04745 971079..971564 + 486 WP_002296241.1 single-stranded DNA-binding protein -
E6A31_RS04750 971930..972190 + 261 WP_002296240.1 hypothetical protein -
E6A31_RS04755 972631..972837 + 207 WP_002296239.1 hypothetical protein -
E6A31_RS04760 972837..973088 + 252 WP_002296238.1 hypothetical protein -
E6A31_RS04765 973103..973540 + 438 WP_002296237.1 hypothetical protein -
E6A31_RS04770 973533..974240 + 708 WP_002353391.1 hypothetical protein -
E6A31_RS04775 974621..974800 + 180 WP_002305761.1 hypothetical protein -
E6A31_RS04780 975141..975404 + 264 WP_002287514.1 hypothetical protein Antitoxin
E6A31_RS04785 975398..975748 + 351 WP_002321032.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E6A31_RS04790 975772..976386 - 615 WP_010729795.1 recombinase family protein -
E6A31_RS04795 976700..977122 + 423 WP_002303116.1 hypothetical protein -
E6A31_RS04800 977132..977335 + 204 WP_002299573.1 hypothetical protein -
E6A31_RS04805 977537..977821 + 285 Protein_940 recombinase family protein -
E6A31_RS04810 977896..978576 + 681 WP_010861579.1 IS6-like element IS1216 family transposase -
E6A31_RS04815 978765..979085 - 321 Protein_942 helix-turn-helix domain-containing protein -
E6A31_RS04820 979136..979816 - 681 WP_010861579.1 IS6-like element IS1216 family transposase -
E6A31_RS04825 979887..980624 + 738 Protein_944 peptidoglycan-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-111)

Antitoxin

Antitoxin PemI-like

(4-87)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 13255.28 Da        Isoelectric Point: 8.0280

>T123434 WP_002321032.1 NZ_CP038996:975398-975748 [Enterococcus faecium]
MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVVTVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLT
TIDYEARECIFLEKAHDSLVEELLMKVRTVFQKVSK

Download         Length: 351 bp

>T123434 NZ_CP038996:975398-975748 [Enterococcus faecium]
ATGGTGAGAAAACCTAGACAAGGCGATATTCTATTATTGAATACAGCACCTCGATCTGGACACGAACAGACAGGTAAAAG
ACCTTATATTGTTTTAAGTCATGATATTATTGCGGATTATAGTAACGTTGTTACAGTTGCTCCAATTTCTAGTACAACTA
GAAACTATCCGCTATATGTAAATATAAACTCAGAATATGAAATGAAGACAACAGGAAAAGTATTATTAGATCAACTAACG
ACAATTGATTATGAAGCCAGAGAGTGTATTTTTTTGGAAAAAGCGCATGATAGTTTAGTTGAGGAGTTATTGATGAAAGT
TAGAACAGTTTTTCAAAAAGTAAGCAAATAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 10183.60 Da        Isoelectric Point: 4.6865

>AT123434 WP_002287514.1 NZ_CP038996:975141-975404 [Enterococcus faecium]
MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQKRLIIEAANIENELTIEQLFENYTDERNHVTIQNLGE
AVGNEKW

Download         Length: 264 bp

>AT123434 NZ_CP038996:975141-975404 [Enterococcus faecium]
ATGGAAATGTTACCTATAAAAAAATGGGGAAATAGTAATGGCCTTAGATTGCCAAAGTATATTATGGAATATCTTGGAAT
TCATACAGAAGACAAAGTGAAGATAATACAAGAAGAAGTAAACGGTCAGAAGCGGCTAATTATCGAAGCGGCTAATATAG
AAAATGAATTAACAATTGAACAGTTATTTGAAAATTACACTGATGAAAGAAATCATGTGACGATTCAAAATTTAGGTGAA
GCAGTAGGTAATGAAAAATGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F5H8

Antitoxin

Source ID Structure
AlphaFold DB A0A829F3C0

References