Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 478140..478711 Replicon chromosome
Accession NZ_CP038996
Organism Enterococcus faecium strain SRR24

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag E6A31_RS02260 Protein ID WP_002286801.1
Coordinates 478370..478711 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag E6A31_RS02255 Protein ID WP_002323011.1
Coordinates 478140..478370 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6A31_RS02230 473621..474949 + 1329 WP_156264942.1 FAD-dependent oxidoreductase -
E6A31_RS02235 474971..475597 + 627 WP_002286816.1 cysteine hydrolase -
E6A31_RS02240 475780..476361 + 582 WP_002331663.1 TetR/AcrR family transcriptional regulator -
E6A31_RS02245 476726..477301 + 576 WP_002331662.1 SOS response-associated peptidase family protein -
E6A31_RS02250 477506..477844 - 339 WP_044383733.1 hypothetical protein -
E6A31_RS02255 478140..478370 + 231 WP_002323011.1 hypothetical protein Antitoxin
E6A31_RS02260 478370..478711 + 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E6A31_RS02265 479561..479746 + 186 WP_002304207.1 hypothetical protein -
E6A31_RS02270 480016..481178 + 1163 WP_086956687.1 IS3 family transposase -
E6A31_RS02275 481426..481551 + 126 WP_025478972.1 LPXTG cell wall anchor domain-containing protein -
E6A31_RS02280 481625..482521 + 897 WP_002352497.1 class C sortase -
E6A31_RS02285 482702..483376 + 675 WP_002291233.1 response regulator transcription factor -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T123433 WP_002286801.1 NZ_CP038996:478370-478711 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T123433 NZ_CP038996:478370-478711 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT123433 WP_002323011.1 NZ_CP038996:478140-478370 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT123433 NZ_CP038996:478140-478370 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References