Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 303850..304421 Replicon chromosome
Accession NZ_CP038996
Organism Enterococcus faecium strain SRR24

Toxin (Protein)


Gene name mazF Uniprot ID A0A2W4HYX0
Locus tag E6A31_RS01480 Protein ID WP_002315988.1
Coordinates 304080..304421 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2A4E1M8
Locus tag E6A31_RS01475 Protein ID WP_025477367.1
Coordinates 303850..304080 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E6A31_RS01460 300122..301561 - 1440 Protein_291 helix-turn-helix domain-containing protein -
E6A31_RS01465 301705..302025 + 321 Protein_292 MmcQ/YjbR family DNA-binding protein -
E6A31_RS01470 302136..303595 + 1460 Protein_293 IS1182-like element ISEfa7 family transposase -
E6A31_RS01475 303850..304080 + 231 WP_025477367.1 hypothetical protein Antitoxin
E6A31_RS01480 304080..304421 + 342 WP_002315988.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E6A31_RS01485 305116..307197 + 2082 WP_002315986.1 BglG family transcription antiterminator -
E6A31_RS01490 307233..307535 + 303 WP_002292534.1 PTS sugar transporter subunit IIB -
E6A31_RS01495 307561..308850 + 1290 WP_002315985.1 PTS sugar transporter subunit IIC -
E6A31_RS01500 308869..309276 + 408 WP_002315984.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13330.69 Da        Isoelectric Point: 9.9258

>T123432 WP_002315988.1 NZ_CP038996:304080-304421 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSIGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVLISQ
LKSLDFKERRLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T123432 NZ_CP038996:304080-304421 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTGTGGATTGATTTTGATCCTTCGATAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAGTTTAATCGTAAAACAATGTTTTCGGTCATATGTCCGATTA
CTTCCACGATAAAGAATTTACCGACACGTTACTCATTACCAGAAGAGCTAGATATAAAAGGACAAGTACTTATTTCTCAA
TTAAAATCACTTGATTTTAAGGAACGAAGACTCAAAAAAATAGAAAACCTGCCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAATATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8566.42 Da        Isoelectric Point: 4.0070

>AT123432 WP_025477367.1 NZ_CP038996:303850-304080 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGRERL

Download         Length: 231 bp

>AT123432 NZ_CP038996:303850-304080 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTTACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTATATTCTGACGATGGGACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GAGAAGAGGCCGAATATTATGAAAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAAGGTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2W4HYX0

Antitoxin

Source ID Structure
AlphaFold DB A0A2A4E1M8

References