Detailed information of TA system TA123413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 49871..50514
• Replicon: plasmid unnamed2
• Accession: NZ_CP038974
• Organism: Piscirickettsia salmonis strain Psal-069

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: Psal069_RS17830
• Protein ID: WP_016210974.1
• Coordinates: 50107..50514 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: Psal069_RS17825
• Protein ID: WP_016210972.1
• Coordinates: 49871..50098 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal069_RS17770	44967..45272	+	306	WP_016210958.1	hypothetical protein	-
Psal069_RS17775	45426..45725	+	300	WP_016210976.1	hypothetical protein	-
Psal069_RS17780	45772..46587	+	816	WP_155049212.1	hypothetical protein	-
Psal069_RS17785	46610..47170	+	561	WP_155049214.1	hypothetical protein	-
Psal069_RS17790	47139..47501	+	363	WP_016210959.1	hypothetical protein	-
Psal069_RS17795	47494..47841	+	348	WP_016210966.1	hypothetical protein	-
Psal069_RS17800	47838..48077	+	240	WP_016210963.1	hypothetical protein	-
Psal069_RS17805	48173..48460	+	288	WP_036794065.1	hypothetical protein	-
Psal069_RS17810	48457..48741	+	285	WP_036794070.1	hypothetical protein	-
Psal069_RS17815	48743..49216	+	474	WP_016210960.1	single-stranded DNA-binding protein	-
Psal069_RS17820	49331..49723	+	393	WP_129556726.1	hypothetical protein	-
Psal069_RS17825	49871..50098	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal069_RS17830	50107..50514	+	408	WP_016210974.1	DUF4411 family protein	Toxin
Psal069_RS17835	50631..51311	+	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
Psal069_RS17840	51490..51783	+	294	WP_016210977.1	zinc ribbon domain-containing protein	-
Psal069_RS17845	52001..52183	-	183	WP_129556724.1	hypothetical protein	-
Psal069_RS17850	52328..52507	-	180	WP_155046773.1	phosphatase	-
Psal069_RS17855	52602..52919	+	318	WP_129556723.1	hypothetical protein	-
Psal069_RS17860	53220..53603	+	384	WP_032126134.1	HNH endonuclease	-
Psal069_RS17865	53691..54170	+	480	WP_016212234.1	P27 family phage terminase small subunit	-
Psal069_RS17870	54174..54383	+	210	WP_016212231.1	hypothetical protein	-
Psal069_RS17875	54438..54755	+	318	WP_164997282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..78730	78730

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123413 WP_016210974.1 NZ_CP038974:50107-50514 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123413 NZ_CP038974:50107-50514 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT123413 WP_016210972.1 NZ_CP038974:49871-50098 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123413 NZ_CP038974:49871-50098 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5