Detailed information of TA system TA123409

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 36447..37090
• Replicon: plasmid unnamed2
• Accession: NZ_CP038969
• Organism: Piscirickettsia salmonis strain Psal-068

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: Psal068_RS17525
• Protein ID: WP_016210974.1
• Coordinates: 36683..37090 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: Psal068_RS17520
• Protein ID: WP_016210972.1
• Coordinates: 36447..36674 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal068_RS17465	31543..31848	+	306	WP_016210958.1	hypothetical protein	-
Psal068_RS17470	32002..32301	+	300	WP_016210976.1	hypothetical protein	-
Psal068_RS17475	32348..33163	+	816	WP_016210962.1	hypothetical protein	-
Psal068_RS17480	33186..33746	+	561	WP_016210973.1	hypothetical protein	-
Psal068_RS17485	33715..34077	+	363	WP_016210959.1	hypothetical protein	-
Psal068_RS17490	34070..34417	+	348	WP_016210966.1	hypothetical protein	-
Psal068_RS17495	34414..34653	+	240	WP_016210963.1	hypothetical protein	-
Psal068_RS17500	34749..35036	+	288	WP_036794065.1	hypothetical protein	-
Psal068_RS17505	35033..35317	+	285	WP_036794070.1	hypothetical protein	-
Psal068_RS17510	35319..35792	+	474	WP_016210960.1	single-stranded DNA-binding protein	-
Psal068_RS17515	35907..36299	+	393	WP_129556726.1	hypothetical protein	-
Psal068_RS17520	36447..36674	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal068_RS17525	36683..37090	+	408	WP_016210974.1	DUF4411 family protein	Toxin
Psal068_RS17530	37207..37887	+	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
Psal068_RS17535	38066..38359	+	294	WP_016210977.1	zinc ribbon domain-containing protein	-
Psal068_RS17540	38577..38759	-	183	WP_129556724.1	hypothetical protein	-
Psal068_RS17545	38904..39083	-	180	WP_155046773.1	phosphatase	-
Psal068_RS17550	39178..39495	+	318	WP_129556723.1	hypothetical protein	-
Psal068_RS17555	39796..40179	+	384	WP_032126134.1	HNH endonuclease	-
Psal068_RS17560	40267..40746	+	480	WP_016212234.1	P27 family phage terminase small subunit	-
Psal068_RS17565	40750..40959	+	210	WP_016212231.1	hypothetical protein	-
Psal068_RS17570	41014..41331	+	318	WP_164997282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..79943	79943

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123409 WP_016210974.1 NZ_CP038969:36683-37090 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123409 NZ_CP038969:36683-37090 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT123409 WP_016210972.1 NZ_CP038969:36447-36674 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123409 NZ_CP038969:36447-36674 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5