Detailed information of TA system TA123401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 22163..22806
• Replicon: plasmid unnamed3
• Accession: NZ_CP038960
• Organism: Piscirickettsia salmonis strain Psal-041

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: Psal041_RS17765
• Protein ID: WP_016210974.1
• Coordinates: 22163..22570 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: Psal041_RS17770
• Protein ID: WP_016210972.1
• Coordinates: 22579..22806 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal041_RS17730	17409..17888	-	480	WP_016212234.1	P27 family phage terminase small subunit	-
Psal041_RS17735	17965..18357	-	393	WP_155047322.1	HNH endonuclease	-
Psal041_RS17740	18692..19666	-	975	WP_054300271.1	IS30 family transposase	-
Psal041_RS17745	19764..20081	-	318	WP_129556723.1	hypothetical protein	-
Psal041_RS17750	20282..20611	-	330	WP_036780870.1	hypothetical protein	-
Psal041_RS17755	20611..20937	-	327	WP_155047323.1	zinc ribbon domain-containing protein	-
Psal041_RS17760	21366..22151	-	786	WP_155047324.1	DNA/RNA non-specific endonuclease	-
Psal041_RS17765	22163..22570	-	408	WP_016210974.1	DUF4411 family protein	Toxin
Psal041_RS17770	22579..22806	-	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal041_RS17775	23102..24076	+	975	WP_054300271.1	IS30 family transposase	-
Psal041_RS17780	24096..24425	-	330	WP_155047330.1	hypothetical protein	-
Psal041_RS17785	24567..25040	-	474	WP_016210960.1	single-stranded DNA-binding protein	-
Psal041_RS17790	25042..25326	-	285	WP_036794070.1	hypothetical protein	-
Psal041_RS17795	25323..25712	-	390	WP_036780969.1	hypothetical protein	-
Psal041_RS17800	25705..25944	-	240	WP_155047325.1	hypothetical protein	-
Psal041_RS17805	25941..26288	-	348	WP_155047331.1	hypothetical protein	-
Psal041_RS17810	26281..26643	-	363	WP_016210959.1	hypothetical protein	-
Psal041_RS17815	26612..27172	-	561	WP_016210973.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..38426	38426

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123401 WP_016210974.1 NZ_CP038960:c22570-22163 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123401 NZ_CP038960:c22570-22163 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT123401 WP_016210972.1 NZ_CP038960:c22806-22579 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123401 NZ_CP038960:c22806-22579 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5