Detailed information of TA system TA123378

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 5734..6340
• Replicon: plasmid unnamed3
• Accession: NZ_CP038930
• Organism: Piscirickettsia salmonis strain Psal-013

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: Psal013_RS17765
• Protein ID: WP_027242955.1
• Coordinates: 5734..5994 (+)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A7U4WZG4
• Locus tag: Psal013_RS17770
• Protein ID: WP_016211078.1
• Coordinates: 5987..6340 (+)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal013_RS17735	994..1977	+	984	WP_016211499.1	ParB/RepB/Spo0J family partition protein	-
Psal013_RS18700	2483..2758	+	276	WP_171824216.1	hypothetical protein	-
Psal013_RS17745	2963..3406	+	444	WP_178099494.1	transposase	-
Psal013_RS17750	3403..4377	-	975	WP_036771330.1	IS30 family transposase	-
Psal013_RS18705	4491..4847	+	357	WP_075278739.1	transposase	-
Psal013_RS17760	4913..5503	-	591	WP_016212329.1	site-specific integrase	-
Psal013_RS17765	5734..5994	+	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
Psal013_RS17770	5987..6340	+	354	WP_016211078.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Psal013_RS17775	6517..7491	+	975	WP_069971681.1	IS30 family transposase	-
Psal013_RS17780	7600..7827	+	228	Protein_10	hypothetical protein	-
Psal013_RS17785	8024..8389	+	366	WP_027242954.1	hypothetical protein	-
Psal013_RS17790	8534..8788	+	255	WP_027242953.1	type II toxin-antitoxin system HicA family toxin	-
Psal013_RS17795	8781..9128	+	348	WP_027242952.1	type II toxin-antitoxin system HicB family antitoxin	-
Psal013_RS17800	9226..10200	+	975	WP_036771330.1	IS30 family transposase	-
Psal013_RS18710	10432..10578	-	147	WP_169834793.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..33555	33555

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T123378 WP_027242955.1 NZ_CP038930:5734-5994 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T123378 NZ_CP038930:5734-5994 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTCATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13492.39 Da
• Isoelectric Point: 5.0200

>AT123378 WP_016211078.1 NZ_CP038930:5987-6340 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQTVKR

Nucleotide

• Length: 354 bp

>AT123378 NZ_CP038930:5987-6340 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGACTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTAGA
AAAGTTGAAGCATGATCAAACCGTTAAACGGTGA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87

Antitoxin

• AlphaFold DB: A0A7U4WZG4