Detailed information of TA system TA123374

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2697557..2698320
• Replicon: chromosome
• Accession: NZ_CP038927
• Organism: Piscirickettsia salmonis strain Psal-013

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Psal013_RS13755
• Protein ID: WP_144420759.1
• Coordinates: 2697557..2698057 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: Psal013_RS13760
• Protein ID: WP_017376501.1
• Coordinates: 2698054..2698320 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal013_RS13725	2692569..2693684	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
Psal013_RS13730	2693847..2694371	+	525	WP_017376000.1	inorganic diphosphatase	-
Psal013_RS13735	2694371..2694901	+	531	WP_017375999.1	transcriptional repressor	-
Psal013_RS13740	2695232..2695471	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
Psal013_RS18585	2695396..2696058	+	663	WP_174872532.1	transposase	-
Psal013_RS18590	2696062..2696367	+	306	WP_146619445.1	transposase	-
Psal013_RS13750	2696561..2697436	+	876	WP_048875904.1	IS982 family transposase	-
Psal013_RS13755	2697557..2698057	-	501	WP_144420759.1	GNAT family N-acetyltransferase	Toxin
Psal013_RS13760	2698054..2698320	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
Psal013_RS13765	2698486..2699460	-	975	WP_048875903.1	IS30 family transposase	-
Psal013_RS13770	2699640..2700260	-	621	WP_075275269.1	transposase	-
Psal013_RS13775	2700257..2700553	-	297	Protein_2704	transposase	-
Psal013_RS13780	2700567..2701970	-	1404	WP_048876031.1	IS4 family transposase	-
Psal013_RS13785	2702094..2702681	-	588	Protein_2706	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2696561..2702681	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18558.59 Da
• Isoelectric Point: 9.0858

>T123374 WP_144420759.1 NZ_CP038927:c2698057-2697557 [Piscirickettsia salmonis]
ITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPETIP
VIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKIRQL
LKNETK

Nucleotide

• Length: 501 bp

>T123374 NZ_CP038927:c2698057-2697557 [Piscirickettsia salmonis]
ATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAATGAGTGGTT
ACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAGTGATTGGCT
ATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAGACAATTCCG
GTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAAAGATGCTTT
AATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAGCAAAACAGT
TTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATTAGGCAACTT
CTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT123374 WP_017376501.1 NZ_CP038927:c2698320-2698054 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT123374 NZ_CP038927:c2698320-2698054 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L6TGZ1