Detailed information of TA system TA123364

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2673411..2674174
• Replicon: chromosome
• Accession: NZ_CP038918
• Organism: Piscirickettsia salmonis strain Psal-010b

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Psal010b_RS13565
• Protein ID: WP_144420759.1
• Coordinates: 2673411..2673911 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: Psal010b_RS13570
• Protein ID: WP_017376501.1
• Coordinates: 2673908..2674174 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal010b_RS13535	2668423..2669538	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
Psal010b_RS13540	2669701..2670225	+	525	WP_017376000.1	inorganic diphosphatase	-
Psal010b_RS13545	2670225..2670755	+	531	WP_017375999.1	transcriptional repressor	-
Psal010b_RS13550	2671086..2671325	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
Psal010b_RS18615	2671250..2671912	+	663	WP_174872532.1	transposase	-
Psal010b_RS18620	2671916..2672221	+	306	WP_146619445.1	transposase	-
Psal010b_RS13560	2672415..2673290	+	876	WP_048875904.1	IS982 family transposase	-
Psal010b_RS13565	2673411..2673911	-	501	WP_144420759.1	GNAT family N-acetyltransferase	Toxin
Psal010b_RS13570	2673908..2674174	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
Psal010b_RS13575	2674340..2675314	-	975	WP_048875903.1	IS30 family transposase	-
Psal010b_RS13580	2675494..2676114	-	621	WP_075275269.1	transposase	-
Psal010b_RS13585	2676111..2676407	-	297	Protein_2671	transposase	-
Psal010b_RS13590	2676421..2677824	-	1404	WP_048876031.1	IS4 family transposase	-
Psal010b_RS13595	2677948..2678535	-	588	Protein_2673	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2672415..2678535	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18558.59 Da
• Isoelectric Point: 9.0858

>T123364 WP_144420759.1 NZ_CP038918:c2673911-2673411 [Piscirickettsia salmonis]
ITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPETIP
VIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKIRQL
LKNETK

Nucleotide

• Length: 501 bp

>T123364 NZ_CP038918:c2673911-2673411 [Piscirickettsia salmonis]
ATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAATGAGTGGTT
ACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAGTGATTGGCT
ATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAGACAATTCCG
GTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAAAGATGCTTT
AATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAGCAAAACAGT
TTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATTAGGCAACTT
CTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT123364 WP_017376501.1 NZ_CP038918:c2674174-2673908 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT123364 NZ_CP038918:c2674174-2673908 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L6TGZ1