Detailed information of TA system TA123351

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 2679013..2679776
• Replicon: chromosome
• Accession: NZ_CP038904
• Organism: Piscirickettsia salmonis strain Psal-008

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: Psal008_RS13590
• Protein ID: WP_144420759.1
• Coordinates: 2679013..2679513 (-)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A1L6TGZ1
• Locus tag: Psal008_RS13595
• Protein ID: WP_017376501.1
• Coordinates: 2679510..2679776 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal008_RS13560	2674025..2675140	+	1116	WP_017376001.1	DSD1 family PLP-dependent enzyme	-
Psal008_RS13565	2675303..2675827	+	525	WP_017376000.1	inorganic diphosphatase	-
Psal008_RS13570	2675827..2676357	+	531	WP_017375999.1	transcriptional repressor	-
Psal008_RS13575	2676688..2676927	+	240	WP_075275277.1	transposase zinc-binding domain-containing protein	-
Psal008_RS18310	2676852..2677514	+	663	WP_174872532.1	transposase	-
Psal008_RS18315	2677518..2677823	+	306	WP_146619445.1	transposase	-
Psal008_RS13585	2678017..2678892	+	876	WP_048875904.1	IS982 family transposase	-
Psal008_RS13590	2679013..2679513	-	501	WP_144420759.1	GNAT family N-acetyltransferase	Toxin
Psal008_RS13595	2679510..2679776	-	267	WP_017376501.1	DUF1778 domain-containing protein	Antitoxin
Psal008_RS13600	2679942..2680916	-	975	WP_048875903.1	IS30 family transposase	-
Psal008_RS13605	2681096..2681716	-	621	WP_075275269.1	transposase	-
Psal008_RS13610	2681713..2682009	-	297	Protein_2682	transposase	-
Psal008_RS13615	2682023..2683426	-	1404	WP_048876031.1	IS4 family transposase	-
Psal008_RS13620	2683550..2684137	-	588	Protein_2684	IS982-like element ISPsa1 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	2678017..2684137	6120

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (67-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (7-81)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18558.59 Da
• Isoelectric Point: 9.0858

>T123351 WP_144420759.1 NZ_CP038904:c2679513-2679013 [Piscirickettsia salmonis]
ITPPELLESSHDTRGFDCGDIALNEWLQKRGLKNQTSDASRVYVITNRHRVIGYYALASGSVERMIVPGSIARNMPETIP
VIVLGRLAVDLDYHDQKLGSGLLKDALIRCMLAADTIAAKAVMVHAISDRAKQFYTKNSFVQCPEIEMTLFISIKKIRQL
LKNETK

Nucleotide

• Length: 501 bp

>T123351 NZ_CP038904:c2679513-2679013 [Piscirickettsia salmonis]
ATAACACCGCCGGAGCTGCTAGAGTCTAGCCATGATACTCGAGGTTTTGATTGCGGAGATATTGCGCTTAATGAGTGGTT
ACAAAAGCGAGGATTAAAAAATCAGACCTCTGATGCAAGTCGAGTGTATGTAATTACTAATCGTCATCGAGTGATTGGCT
ATTATGCGTTAGCCAGTGGCTCTGTGGAGCGAATGATCGTACCTGGCTCCATAGCTCGTAATATGCCAGAGACAATTCCG
GTGATTGTGCTAGGGCGGCTAGCCGTTGACCTTGATTATCACGATCAAAAACTAGGAAGCGGCTTATTAAAAGATGCTTT
AATTCGGTGTATGCTAGCTGCTGATACAATCGCTGCAAAGGCAGTCATGGTTCATGCAATCTCAGATAGAGCAAAACAGT
TTTATACTAAAAACTCTTTTGTCCAATGTCCTGAAATTGAAATGACTTTATTTATTTCTATAAAAAAAATTAGGCAACTT
CTAAAAAATGAAACAAAATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10033.51 Da
• Isoelectric Point: 4.4819

>AT123351 WP_017376501.1 NZ_CP038904:c2679776-2679510 [Piscirickettsia salmonis]
MAKVTPISMRTTAEQFDLLTKAANALNIDRTSFILNIACREAENVLLDRRLFQLNDEAFDAFEAALDEPLTQPEKLKRLL
TEPAPWEK

Nucleotide

• Length: 267 bp

>AT123351 NZ_CP038904:c2679776-2679510 [Piscirickettsia salmonis]
ATGGCAAAGGTAACACCGATTAGCATGCGTACAACAGCGGAACAGTTTGATTTACTTACTAAAGCAGCAAATGCATTAAA
TATAGACCGCACATCGTTCATTTTGAATATTGCTTGCCGTGAAGCTGAAAACGTACTGCTAGACCGCCGTTTGTTTCAAT
TAAATGATGAAGCTTTTGATGCCTTTGAGGCAGCACTAGATGAGCCATTAACTCAACCAGAGAAATTAAAACGCTTACTA
ACGGAGCCTGCACCTTGGGAGAAATAA

Structures

Antitoxin

• AlphaFold DB: A0A1L6TGZ1