Detailed information of TA system TA123342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 20094..20700
• Replicon: plasmid unnamed2
• Accession: NZ_CP038895
• Organism: Piscirickettsia salmonis strain Psal-006a

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0B8UI87
• Locus tag: Psal006a_RS17605
• Protein ID: WP_027242955.1
• Coordinates: 20440..20700 (-)
• Length: 87 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: Psal006a_RS17600
• Protein ID: WP_036780061.1
• Coordinates: 20094..20447 (-)
• Length: 118 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal006a_RS17580	16900..17628	+	729	WP_017375910.1	IS6 family transposase	-
Psal006a_RS17585	17770..18702	+	933	WP_144420837.1	hypothetical protein	-
Psal006a_RS17590	18732..19022	+	291	Protein_22	IS6 family transposase	-
Psal006a_RS17595	19089..19817	-	729	WP_017375910.1	IS6 family transposase	-
Psal006a_RS17600	20094..20447	-	354	WP_036780061.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
Psal006a_RS17605	20440..20700	-	261	WP_027242955.1	type II toxin-antitoxin system HicA family toxin	Toxin
Psal006a_RS17610	20949..21923	+	975	WP_054300271.1	IS30 family transposase	-
Psal006a_RS17615	22100..23332	-	1233	WP_032126623.1	MFS transporter	-
Psal006a_RS17620	23445..24470	-	1026	WP_032126388.1	helix-turn-helix domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57410	57410

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-82)

Antitoxin

• Uncharacterised protein family HicB (53-103)

Sequences

Toxin

Protein

• Length: 87 a.a.
• Molecular weight: 9695.31 Da
• Isoelectric Point: 10.9468

>T123342 WP_027242955.1 NZ_CP038895:c20700-20440 [Piscirickettsia salmonis]
MNNKHRKTLRDIFHNPIKASIVWADIEKLLLALGAEVIERDGSRVAVILNQVKTTFHRPHPEKTTDKGAVKSIRLFLKNA
GVDKNA

Nucleotide

• Length: 261 bp

>T123342 NZ_CP038895:c20700-20440 [Piscirickettsia salmonis]
ATGAATAATAAACATCGTAAGACACTAAGAGATATTTTTCATAACCCGATCAAAGCAAGCATTGTTTGGGCTGATATAGA
AAAGCTTCTTTTAGCGTTAGGGGCTGAAGTAATAGAGCGTGATGGTTCAAGGGTTGCTGTAATTTTGAATCAAGTGAAAA
CAACATTTCATAGACCGCACCCTGAAAAAACAACAGACAAGGGGGCGGTTAAGTCAATAAGGTTGTTTTTAAAAAATGCT
GGAGTAGATAAAAATGCTTAA

Antitoxin

Protein

• Length: 118 a.a.
• Molecular weight: 13504.44 Da
• Isoelectric Point: 5.0200

>AT123342 WP_036780061.1 NZ_CP038895:c20447-20094 [Piscirickettsia salmonis]
MLKYKGYIGKVEEIHEGDEYIYGIVPNIKDVISFRGKNIKELTKDFKLAIDDYLALCDEREEEPDKPFSGRFMTRLGSDL
HEEVFLASMNDSGAKSMNDWVVSACLEKLKHDQIVKR

Nucleotide

• Length: 354 bp

>AT123342 NZ_CP038895:c20447-20094 [Piscirickettsia salmonis]
ATGCTTAAATACAAAGGTTATATTGGAAAAGTTGAAGAGATTCATGAAGGTGATGAATATATTTATGGGATTGTTCCAAA
TATTAAAGATGTTATTTCTTTTAGAGGTAAAAATATAAAAGAGTTAACGAAAGACTTTAAGCTGGCTATTGATGATTATT
TGGCTTTATGTGATGAGCGTGAAGAAGAACCCGATAAACCATTTTCTGGTAGATTTATGACTAGATTAGGTAGTGATTTG
CATGAAGAGGTTTTTTTAGCTTCTATGAATGATTCAGGTGCAAAATCAATGAATGATTGGGTTGTATCTGCATGCTTGGA
AAAGTTGAAGCATGATCAAATCGTTAAACGGTAA

Structures

Toxin

• AlphaFold DB: A0A0B8UI87