Detailed information of TA system TA123326

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 464006..464580
• Replicon: chromosome
• Accession: NZ_CP038875
• Organism: Pasteurella multocida strain FCf83

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: -
• Locus tag: E5138_RS02205
• Protein ID: WP_061406134.1
• Coordinates: 464006..464290 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: E5138_RS02210
• Protein ID: WP_135938169.1
• Coordinates: 464287..464580 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5138_RS02185	459799..461388	-	1590	WP_081106270.1	AAA family ATPase	-
E5138_RS02190	461673..462224	-	552	WP_196766247.1	NERD domain-containing protein	-
E5138_RS02195	462368..462727	+	360	WP_095261631.1	DUF3418 domain-containing protein	-
E5138_RS02205	464006..464290	+	285	WP_061406134.1	hypothetical protein	Toxin
E5138_RS02210	464287..464580	+	294	WP_135938169.1	putative addiction module antidote protein	Antitoxin
E5138_RS02215	464983..465660	-	678	WP_005726756.1	3-keto-L-gulonate-6-phosphate decarboxylase UlaD	-
E5138_RS02220	465678..466145	-	468	WP_064661066.1	PTS sugar transporter subunit IIA	-
E5138_RS02225	466190..467980	-	1791	WP_061406132.1	PTS ascorbate-specific subunit IIBC	-
E5138_RS02230	468330..469421	+	1092	WP_005751571.1	L-ascorbate 6-phosphate lactonase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (4-87)

Antitoxin

• Uncharacterized protein HI1420 (5-92)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10667.40 Da
• Isoelectric Point: 10.2297

>T123326 WP_061406134.1 NZ_CP038875:464006-464290 [Pasteurella multocida]
VGRLKEVKILSAKSAVLARINKAMNGNFGDHKSIGNGLYEMRIIKGSGYRVYYGQYREVTYLLICGGDKSTQKSDIVKAR
ELWKEIKQQEEVKV

Nucleotide

• Length: 285 bp

>T123326 NZ_CP038875:464006-464290 [Pasteurella multocida]
GTGGGTCGGTTAAAGGAGGTAAAAATTTTATCCGCCAAATCTGCTGTTCTTGCTCGTATTAACAAAGCAATGAATGGTAA
TTTTGGCGATCATAAATCGATTGGAAATGGATTGTATGAGATGAGAATTATAAAAGGTTCGGGTTATAGAGTTTACTATG
GACAATATAGAGAAGTAACTTATTTATTAATTTGTGGTGGCGATAAATCAACACAGAAATCAGACATTGTTAAAGCGAGA
GAGTTGTGGAAAGAAATAAAGCAACAAGAGGAAGTAAAAGTATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10948.83 Da
• Isoelectric Point: 7.2766

>AT123326 WP_135938169.1 NZ_CP038875:464287-464580 [Pasteurella multocida]
MSKKKIKLKLFDIAEHLDSEEMIAAYLSEVLETCDTNEFISALSDVVRAKGMTELAAKTGLRRESLYKTLSYGSEPRFDT
IMKIMHSLGIKLVPTHI

Nucleotide

• Length: 294 bp

>AT123326 NZ_CP038875:464287-464580 [Pasteurella multocida]
ATGAGTAAGAAAAAAATAAAATTAAAACTGTTTGATATTGCTGAACATCTAGACAGTGAAGAAATGATTGCGGCATATTT
AAGTGAAGTTTTAGAAACATGTGATACTAATGAATTTATTTCCGCTTTAAGTGATGTTGTAAGAGCAAAAGGAATGACGG
AGTTAGCAGCAAAAACAGGGTTAAGAAGAGAAAGCCTCTATAAAACATTATCATACGGTAGCGAGCCACGTTTTGATACG
ATAATGAAAATTATGCATTCCTTAGGTATTAAACTTGTCCCAACGCACATCTAA