Detailed information of TA system TA123322

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): VapXD/-
• Location: 1055003..1055477
• Replicon: chromosome
• Accession: NZ_CP038873
• Organism: Pasteurella multocida strain FCf45

Toxin (Protein)

• Gene name: VapD
• Uniprot ID: -
• Locus tag: E5135_RS04805
• Protein ID: WP_135953125.1
• Coordinates: 1055196..1055477 (+)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: VapX
• Uniprot ID: -
• Locus tag: E5135_RS04800
• Protein ID: WP_135953124.1
• Coordinates: 1055003..1055191 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5135_RS04765	1050418..1051839	-	1422	WP_135953120.1	TIGR03752 family integrating conjugative element protein	-
E5135_RS04770	1051826..1052740	-	915	WP_135953121.1	TIGR03749 family integrating conjugative element protein	-
E5135_RS04775	1052741..1053379	-	639	WP_065189262.1	TIGR03746 family integrating conjugative element protein	-
E5135_RS04780	1053380..1053748	-	369	WP_065189261.1	TIGR03750 family conjugal transfer protein	-
E5135_RS04785	1053791..1054201	-	411	WP_135953122.1	DUF2976 domain-containing protein	-
E5135_RS04790	1054204..1054452	-	249	WP_065189259.1	DUF3262 family protein	-
E5135_RS04795	1054463..1054780	-	318	WP_204162876.1	RAQPRD family integrative conjugative element protein	-
E5135_RS04800	1055003..1055191	+	189	WP_135953124.1	DUF5397 family protein	Antitoxin
E5135_RS04805	1055196..1055477	+	282	WP_135953125.1	virulence factor	Toxin
E5135_RS04810	1055493..1055675	-	183	WP_135953126.1	hypothetical protein	-
E5135_RS04815	1055797..1056486	-	690	WP_065189256.1	TIGR03747 family integrating conjugative element membrane protein	-
E5135_RS04820	1056483..1056821	-	339	WP_135953127.1	hemophilus-specific protein	-
E5135_RS04825	1056818..1059043	-	2226	WP_135953128.1	type IV conjugative transfer system coupling protein TraD	-
E5135_RS04830	1059043..1059522	-	480	WP_135953171.1	integrating conjugative element protein	-
E5135_RS04835	1059766..1060227	-	462	WP_135953129.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Virulence-associated protein D / CRISPR associated protein Cas2 (1-86)

Antitoxin

• Protein of unknown function DUF5397 (5-62)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10776.14 Da
• Isoelectric Point: 4.5535

>T123322 WP_135953125.1 NZ_CP038873:1055196-1055477 [Pasteurella multocida]
MYAISFDLVVADTEENHPKGVTQAYTDIGTTLNRFGFERIQGSLYTNADENMANLFQAMNALKAMEWFPYSVRDIRAFRI
EQWSDFTPVIKEK

Nucleotide

• Length: 282 bp

>T123322 NZ_CP038873:1055196-1055477 [Pasteurella multocida]
ATGTACGCAATTTCATTTGATCTTGTTGTTGCTGACACAGAAGAAAACCACCCGAAGGGGGTTACTCAGGCTTATACTGA
TATAGGAACAACCTTAAACAGGTTTGGCTTTGAACGTATTCAAGGAAGCCTATACACGAATGCGGATGAAAATATGGCGA
ATCTTTTCCAAGCAATGAATGCATTAAAAGCAATGGAATGGTTTCCTTATTCCGTTCGAGATATTCGTGCATTCAGGATT
GAACAATGGTCTGATTTTACACCTGTTATCAAAGAAAAATGA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6838.84 Da
• Isoelectric Point: 4.8669

>AT123322 WP_135953124.1 NZ_CP038873:1055003-1055191 [Pasteurella multocida]
MQLTEVPTGIVKTFGHFGIPYQVGKPDKALPDGDILVNITLIESGEQQKYRLSKLLNDPNAE

Nucleotide

• Length: 189 bp

>AT123322 NZ_CP038873:1055003-1055191 [Pasteurella multocida]
ATGCAACTTACTGAGGTGCCAACAGGTATTGTTAAAACATTTGGTCATTTTGGTATTCCTTATCAAGTAGGTAAACCTGA
TAAAGCATTACCCGATGGCGATATTTTGGTAAATATCACGTTAATTGAAAGTGGTGAACAACAGAAATATCGATTATCTA
AATTGCTCAATGACCCTAATGCGGAGTAA