Detailed information of TA system TA123316

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 50932..51546
• Replicon: plasmid punnamed2
• Accession: NZ_CP038867
• Organism: Vagococcus sp. CF-49

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: E4Z98_RS09955
• Protein ID: WP_135253541.1
• Coordinates: 51358..51546 (-)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: E4Z98_RS09950
• Protein ID: WP_135253540.1
• Coordinates: 50932..51318 (-)
• Length: 129 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4Z98_RS09935	47647..48687	-	1041	WP_135253537.1	DUF916 and DUF3324 domain-containing protein	-
E4Z98_RS09940	48706..49509	-	804	WP_135253538.1	WxL domain-containing protein	-
E4Z98_RS09945	49626..50480	+	855	WP_135253539.1	helix-turn-helix domain-containing protein	-
E4Z98_RS10105	50604..50780	+	177	WP_167790873.1	putative holin-like toxin	-
E4Z98_RS09950	50932..51318	-	387	WP_135253540.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E4Z98_RS09955	51358..51546	-	189	WP_135253541.1	type II toxin-antitoxin system HicA family toxin	Toxin
E4Z98_RS09960	51644..52105	-	462	WP_135253542.1	hypothetical protein	-
E4Z98_RS09965	52173..53708	-	1536	WP_135253543.1	DUF4942 domain-containing protein	-
E4Z98_RS09970	54267..55613	-	1347	WP_135253544.1	PcfJ domain-containing protein	-
E4Z98_RS09975	55619..56428	-	810	WP_167790874.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..60358	60358

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-61)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (5-106)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7048.46 Da
• Isoelectric Point: 10.8142

>T123316 WP_135253541.1 NZ_CP038867:c51546-51358 [Vagococcus sp. CF-49]
MPLTDREMIKLLVKEGWIIKPKQGKGSHTKLTKEGHRRPIIVPKGELKYGTERSILKEAGLL

Nucleotide

• Length: 189 bp

>T123316 NZ_CP038867:c51546-51358 [Vagococcus sp. CF-49]
TTGCCACTTACAGATAGAGAAATGATTAAACTTTTGGTAAAAGAAGGATGGATAATCAAACCGAAACAAGGTAAAGGTTC
TCATACTAAATTAACCAAAGAAGGTCATAGACGACCAATCATTGTTCCTAAAGGAGAGTTAAAATATGGAACGGAACGCT
CAATTTTGAAAGAAGCAGGACTTTTATAG

Antitoxin

Protein

• Length: 129 a.a.
• Molecular weight: 14459.22 Da
• Isoelectric Point: 4.1075

>AT123316 WP_135253540.1 NZ_CP038867:c51318-50932 [Vagococcus sp. CF-49]
MKKIYPAVFSEEEAGGYYIQFPDLEGNGTQGDTLEEGLDFASDYLGIVLSDLVEHNESLPKPSNIKDISVKDDEFVTLIE
VDLSEYIQDNRLSRTNTNIPVWLKTRAEKQGINFSKLLTEALMNKLEV

Nucleotide

• Length: 387 bp

>AT123316 NZ_CP038867:c51318-50932 [Vagococcus sp. CF-49]
ATGAAAAAAATCTACCCTGCTGTATTTTCAGAAGAAGAAGCTGGTGGTTACTACATTCAATTTCCTGATTTAGAAGGTAA
TGGAACACAAGGTGATACATTAGAAGAAGGTTTAGATTTTGCCTCAGATTATCTAGGTATTGTCTTATCTGACTTAGTGG
AACATAACGAATCACTTCCTAAACCTTCAAATATTAAAGACATTTCTGTAAAAGATGATGAATTTGTGACGTTGATTGAA
GTTGATTTATCAGAGTATATTCAAGATAATAGATTATCTAGAACTAACACCAATATACCTGTATGGTTAAAAACAAGAGC
AGAAAAGCAAGGAATTAATTTTTCCAAGCTATTGACTGAAGCGTTAATGAATAAATTAGAAGTTTAA