Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 21585..22722 Replicon plasmid punnamed1
Accession NZ_CP038866
Organism Vagococcus sp. CF-49

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag E4Z98_RS09650 Protein ID WP_001284311.1
Coordinates 21859..22722 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag E4Z98_RS09645 Protein ID WP_000301765.1
Coordinates 21585..21857 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4Z98_RS10085 17331..17501 + 171 WP_000713594.1 hypothetical protein -
E4Z98_RS09625 17515..18132 + 618 WP_001062589.1 recombinase family protein -
E4Z98_RS09630 18132..20276 + 2145 WP_000108741.1 type IA DNA topoisomerase -
E4Z98_RS09635 20365..21261 + 897 WP_001835294.1 ParA family protein -
E4Z98_RS09640 21359..21568 + 210 WP_000527318.1 peptide-binding protein -
E4Z98_RS09645 21585..21857 + 273 WP_000301765.1 antitoxin Antitoxin
E4Z98_RS09650 21859..22722 + 864 WP_001284311.1 toxin zeta Toxin
E4Z98_RS09655 22985..23722 - 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
E4Z98_RS09660 23847..23930 - 84 WP_031929417.1 23S rRNA methyltransferase attenuator leader peptide ErmL -
E4Z98_RS09670 24714..25031 + 318 WP_002300567.1 hypothetical protein -
E4Z98_RS09680 25565..26062 + 498 WP_002300565.1 molecular chaperone DnaJ -
E4Z98_RS09685 26096..26401 + 306 WP_010711855.1 hypothetical protein -
E4Z98_RS09695 26685..26984 - 300 WP_002301627.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid erm(B) - 1..27416 27416


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T123315 WP_001284311.1 NZ_CP038866:21859-22722 [Vagococcus sp. CF-49]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T123315 NZ_CP038866:21859-22722 [Vagococcus sp. CF-49]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT123315 WP_000301765.1 NZ_CP038866:21585-21857 [Vagococcus sp. CF-49]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT123315 NZ_CP038866:21585-21857 [Vagococcus sp. CF-49]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References