123311

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type VII Classification (family/domain) HepT-MntA/HepT(toxin)
Location 1645107..1645923 Replicon chromosome
Accession NZ_CP038860
Organism Geobacillus kaustophilus NBRC 102445

Toxin (Protein)


Gene name hepT Uniprot ID A0A4D7Q970
Locus tag E5Z46_RS08600 Protein ID WP_042381319.1
Coordinates 1645107..1645526 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name mntA Uniprot ID A0A4D7Q5V4
Locus tag E5Z46_RS08605 Protein ID WP_042381320.1
Coordinates 1645516..1645923 (-) Length 136 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Z46_RS08580 1642017..1642535 + 519 WP_011230465.1 GNAT family N-acetyltransferase -
E5Z46_RS08585 1642664..1643392 + 729 WP_023634217.1 ABC transporter ATP-binding protein -
E5Z46_RS08590 1643389..1644414 + 1026 WP_023634218.1 ABC transporter permease -
E5Z46_RS08595 1644426..1645079 + 654 WP_023634219.1 TetR/AcrR family transcriptional regulator -
E5Z46_RS08600 1645107..1645526 - 420 WP_042381319.1 DUF86 domain-containing protein Toxin
E5Z46_RS08605 1645516..1645923 - 408 WP_042381320.1 nucleotidyltransferase domain-containing protein Antitoxin
E5Z46_RS08610 1646204..1648339 - 2136 WP_042381321.1 ATP-dependent Clp protease ATP-binding subunit -
E5Z46_RS08615 1648607..1649353 + 747 WP_025039183.1 FixH family protein -
E5Z46_RS08620 1649461..1650501 + 1041 WP_023634222.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease HepT-like

(14-131)

Antitoxin

Polymerase beta, nucleotidyltransferase

(9-98)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 15974.39 Da        Isoelectric Point: 6.8345

>T123311 WP_042381319.1 NZ_CP038860:c1645526-1645107 [Geobacillus kaustophilus NBRC 102445]
MKNDVIFNKISIIERCLKRIDEEYDHDPKNLQNDTKQDSIVLNLQRACEACIDLAMHIVAEQKFGLPQHSRDAFALLEEH
GVISPSISKKMKAMVGFRNIAVHDYQQLNLGILQAIVEHHLDDFKQFTKAILDYAKKNS

Download         Length: 420 bp

>T123311 NZ_CP038860:c1645526-1645107 [Geobacillus kaustophilus NBRC 102445]
ATGAAAAATGATGTGATTTTTAATAAGATCAGCATCATTGAACGATGCCTGAAACGAATTGACGAGGAATATGACCACGA
CCCAAAAAATTTACAAAATGACACAAAACAAGATTCGATCGTTCTGAATTTACAGCGGGCGTGCGAGGCATGCATCGATT
TGGCCATGCATATTGTGGCCGAGCAGAAATTCGGATTGCCGCAGCATAGCCGCGATGCATTCGCTCTCCTCGAAGAACAT
GGGGTCATCTCCCCTTCCATAAGCAAAAAGATGAAGGCGATGGTCGGATTCCGCAACATCGCCGTTCACGACTATCAACA
ACTGAACCTTGGCATCTTGCAAGCCATCGTCGAACACCATCTTGATGATTTCAAACAATTTACGAAAGCCATCCTCGATT
ATGCTAAGAAAAACAGCTAG

Antitoxin


Download         Length: 136 a.a.        Molecular weight: 15192.35 Da        Isoelectric Point: 5.6462

>AT123311 WP_042381320.1 NZ_CP038860:c1645923-1645516 [Geobacillus kaustophilus NBRC 102445]
MPNEMETIIIQTLRPALHPFVIYLFGSAARGALHPDSDVDIAFVSDGEPHDPYELFRLAGHLADKLGRDVDLVDLRQAST
VFQAQVVSTGKAIDCRDERKRAEFEMKTLKMYAKLNEERATVLKRIAESGSVYEK

Download         Length: 408 bp

>AT123311 NZ_CP038860:c1645923-1645516 [Geobacillus kaustophilus NBRC 102445]
TTGCCAAACGAAATGGAGACGATCATCATTCAGACGCTCCGCCCGGCTCTTCACCCGTTCGTCATTTATCTGTTCGGTTC
AGCCGCCCGTGGGGCGCTGCACCCAGACAGCGATGTTGATATCGCCTTTGTCAGCGACGGCGAACCGCATGATCCGTATG
AGTTGTTTCGGCTCGCGGGGCATTTGGCTGACAAGTTAGGGCGAGATGTCGATCTTGTCGATTTGCGCCAAGCCAGCACC
GTGTTTCAAGCGCAAGTCGTCTCGACGGGAAAAGCCATTGATTGCCGCGACGAGCGGAAACGGGCTGAGTTCGAAATGAA
AACATTGAAAATGTATGCAAAGCTGAATGAGGAAAGAGCGACGGTGTTAAAACGAATTGCGGAAAGTGGGTCGGTGTATG
AAAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4D7Q970

Antitoxin

Source ID Structure
AlphaFold DB A0A4D7Q5V4

References