123310

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazF-pemI/MazF(toxin)
Location 831484..832120 Replicon chromosome
Accession NZ_CP038860
Organism Geobacillus kaustophilus NBRC 102445

Toxin (Protein)


Gene name mazF Uniprot ID L7ZVP1
Locus tag E5Z46_RS04275 Protein ID WP_003253417.1
Coordinates 831770..832120 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID G8N2B5
Locus tag E5Z46_RS04270 Protein ID WP_011229742.1
Coordinates 831484..831765 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E5Z46_RS04250 827741..828358 - 618 WP_014194682.1 rhomboid family intramembrane serine protease -
E5Z46_RS04255 828472..828861 + 390 WP_011229738.1 holo-ACP synthase -
E5Z46_RS04260 828941..829954 + 1014 WP_014194683.1 outer membrane lipoprotein carrier protein LolA -
E5Z46_RS04265 830203..831357 + 1155 WP_011229741.1 alanine racemase -
E5Z46_RS04270 831484..831765 + 282 WP_011229742.1 hypothetical protein Antitoxin
E5Z46_RS04275 831770..832120 + 351 WP_003253417.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
E5Z46_RS04280 832386..834551 + 2166 WP_042382235.1 RNA-binding transcriptional accessory protein -
E5Z46_RS04285 834570..834683 - 114 WP_013144046.1 cortex morphogenetic protein CmpA -
E5Z46_RS04290 834773..835222 + 450 WP_013144047.1 SprT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12992.00 Da        Isoelectric Point: 4.8781

>T123310 WP_003253417.1 NZ_CP038860:831770-832120 [Geobacillus kaustophilus NBRC 102445]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLGLIDF

Download         Length: 351 bp

>T123310 NZ_CP038860:831770-832120 [Geobacillus kaustophilus NBRC 102445]
TTGATTGTCAAACGTGGCGACGTGTATTTTGCGGACCTTTCCCCGGTTGTTGGCTCGGAGCAGGGCGGCGTGCGCCCCGT
GTTGGTGATCCAAAACGATATCGGCAATCGTTTTAGCCCGACGGTGATTGTCGCGGCGATTACGGCGCAAATCCAAAAAG
CGAAGCTGCCGACGCATGTCGAGATTGACGCGAAACGCTACGGGTTTGAACGCGATTCGGTCATTTTGCTTGAGCAAATT
CGCACGATCGACAAGCAACGGCTGACCGATAAAATCACTCATTTGGACGATGAAATGATGGATAAAGTCGATGAGGCGCT
GCAAATTAGCTTAGGACTGATCGACTTTTGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10639.14 Da        Isoelectric Point: 7.2027

>AT123310 WP_011229742.1 NZ_CP038860:831484-831765 [Geobacillus kaustophilus NBRC 102445]
VSESGATAEIVVRLPQSLLTELDVLVKQENGNRNELIYQATKMYIRERKKRQIREAMRRGYMEMAKINLSIASEAFHAEY
EADHTVERLVSGG

Download         Length: 282 bp

>AT123310 NZ_CP038860:831484-831765 [Geobacillus kaustophilus NBRC 102445]
GTGTCGGAATCTGGCGCAACAGCGGAAATCGTCGTTCGTTTGCCGCAGTCGCTGCTGACAGAACTGGACGTGCTCGTAAA
GCAGGAAAACGGCAACCGCAATGAACTCATTTATCAAGCGACGAAAATGTACATTCGCGAGCGGAAGAAACGGCAAATTC
GCGAGGCGATGAGACGAGGCTACATGGAAATGGCGAAAATCAATTTATCTATCGCTTCTGAAGCGTTTCATGCTGAATAC
GAGGCCGACCACACCGTTGAACGCTTAGTTAGCGGGGGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9ERQ4

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9ETW5

References