Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3158365..3159019 Replicon chromosome
Accession NZ_CP038859
Organism Escherichia coli strain 357900

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag A7A13_RS15235 Protein ID WP_000244772.1
Coordinates 3158365..3158772 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag A7A13_RS15240 Protein ID WP_000354046.1
Coordinates 3158753..3159019 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A13_RS15215 3154322..3156055 - 1734 WP_000813215.1 single-stranded-DNA-specific exonuclease RecJ -
A7A13_RS15220 3156061..3156771 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
A7A13_RS15225 3156796..3157692 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
A7A13_RS15230 3157804..3158325 + 522 WP_001055874.1 flavodoxin FldB -
A7A13_RS15235 3158365..3158772 - 408 WP_000244772.1 protein YgfX Toxin
A7A13_RS15240 3158753..3159019 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
A7A13_RS15245 3159262..3160242 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
A7A13_RS15250 3160319..3160978 - 660 WP_000250274.1 hemolysin III family protein -
A7A13_RS15255 3161142..3161453 - 312 WP_001182944.1 N(4)-acetylcytidine aminohydrolase -
A7A13_RS15260 3161498..3162931 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
A7A13_RS15265 3162988..3163731 - 744 WP_000951964.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T123303 WP_000244772.1 NZ_CP038859:c3158772-3158365 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T123303 NZ_CP038859:c3158772-3158365 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT123303 WP_000354046.1 NZ_CP038859:c3159019-3158753 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT123303 NZ_CP038859:c3159019-3158753 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References