Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907-MazE
Location 3022102..3022685 Replicon chromosome
Accession NZ_CP038859
Organism Escherichia coli strain 357900

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag A7A13_RS14620 Protein ID WP_176670823.1
Coordinates 3022102..3022437 (-) Length 112 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S1P3W5
Locus tag A7A13_RS14625 Protein ID WP_000581937.1
Coordinates 3022437..3022685 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
A7A13_RS14600 3017571..3017933 + 363 WP_000034929.1 hypothetical protein -
A7A13_RS14605 3017989..3019287 - 1299 WP_000036723.1 phosphopyruvate hydratase -
A7A13_RS14610 3019375..3021012 - 1638 WP_000210878.1 CTP synthase (glutamine hydrolyzing) -
A7A13_RS14615 3021240..3022031 - 792 WP_001071648.1 nucleoside triphosphate pyrophosphohydrolase -
A7A13_RS14620 3022102..3022437 - 336 WP_176670823.1 endoribonuclease MazF Toxin
A7A13_RS14625 3022437..3022685 - 249 WP_000581937.1 type II toxin-antitoxin system antitoxin MazE Antitoxin
A7A13_RS14630 3022763..3024997 - 2235 WP_000226815.1 GTP pyrophosphokinase -
A7A13_RS14635 3025045..3026346 - 1302 WP_000046790.1 23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 3013988..3022685 8697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-109)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 112 a.a.        Molecular weight: 12014.97 Da        Isoelectric Point: 8.1170

>T123302 WP_176670823.1 NZ_CP038859:c3022437-3022102 [Escherichia coli]
MVSRYVPDMGDLICVDFDPTKGSEQAGHRPAVVLSPFMYNNKTGMCLCVPCTTQSKGYPFEVVLSGQERDGVALADQVKS
IAWRARGATKKGTVAPEELQLIKAKINVLIG

Download         Length: 336 bp

>T123302 NZ_CP038859:c3022437-3022102 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGTGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9355.74 Da        Isoelectric Point: 4.4665

>AT123302 WP_000581937.1 NZ_CP038859:c3022685-3022437 [Escherichia coli]
MIHSSVKRWGNSPAVRIPATLMQALNLNIDDEVKIDLVDGKLIIEPVRKEPVFTLAELVNDITPENLHENIDWGEPKDKE
VW

Download         Length: 249 bp

>AT123302 NZ_CP038859:c3022685-3022437 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 1UB4
PDB 5CQX
PDB 5CQY
PDB 1MVF
PDB 2MRN
PDB 2MRU
AlphaFold DB A0A7U9LMB4

References