Detailed information of TA system TA123300

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 2657101..2657726
• Replicon: chromosome
• Accession: NZ_CP038859
• Organism: Escherichia coli strain 357900

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: A7A13_RS12755
• Protein ID: WP_000911330.1
• Coordinates: 2657101..2657499 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: A7A13_RS12760
• Protein ID: WP_000450524.1
• Coordinates: 2657499..2657726 (-)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
A7A13_RS12735	2652979..2653179	+	201	WP_000383836.1	YpfN family protein	-
A7A13_RS12740	2653289..2653987	-	699	WP_000679823.1	esterase	-
A7A13_RS12745	2654061..2656076	-	2016	WP_000829329.1	tRNA cytosine(34) acetyltransferase TmcA	-
A7A13_RS12750	2656091..2656954	-	864	WP_001267508.1	neutral zinc metallopeptidase	-
A7A13_RS12755	2657101..2657499	-	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
A7A13_RS12760	2657499..2657726	-	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
A7A13_RS12765	2657880..2658593	-	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
A7A13_RS12770	2658806..2659840	-	1035	WP_001358397.1	outer membrane protein assembly factor BamC	-
A7A13_RS12775	2659857..2660735	-	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
A7A13_RS12780	2660881..2661453	+	573	WP_000176191.1	glycine cleavage system transcriptional repressor	-
A7A13_RS12785	2661453..2661923	+	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2635601..2800699	165098

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T123300 WP_000911330.1 NZ_CP038859:c2657499-2657101 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T123300 NZ_CP038859:c2657499-2657101 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT123300 WP_000450524.1 NZ_CP038859:c2657726-2657499 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT123300 NZ_CP038859:c2657726-2657499 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33