Detailed information of TA system TA123277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): kacAT/ElaA-DUF1778
• Location: 4411..5214
• Replicon: plasmid unnamed
• Accession: NZ_CP038858
• Organism: Escherichia coli strain PigCaeca_2

Toxin (Protein)

• Gene name: KacT
• Uniprot ID: G3CAI8
• Locus tag: E5285_RS23625
• Protein ID: WP_000348883.1
• Coordinates: 4684..5214 (+)
• Length: 177 a.a.

Antitoxin (Protein)

• Gene name: KacA
• Uniprot ID: G3CAI7
• Locus tag: E5285_RS23620
• Protein ID: WP_001275013.1
• Coordinates: 4411..4680 (+)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5285_RS23595	1206..1475	+	270	WP_015060495.1	YacA plasmid stabilization protein	-
E5285_RS23600	1472..1753	+	282	Protein_2	type II toxin-antitoxin system RelE/ParE family toxin	-
E5285_RS23605	1845..2367	-	523	Protein_3	IS1 family transposase	-
E5285_RS23610	2894..3343	+	450	WP_000649995.1	hypothetical protein	-
E5285_RS23615	3459..4156	-	698	Protein_5	IS1 family transposase	-
E5285_RS23620	4411..4680	+	270	WP_001275013.1	DUF1778 domain-containing protein	Antitoxin
E5285_RS23625	4684..5214	+	531	WP_000348883.1	GNAT family N-acetyltransferase	Toxin
E5285_RS23630	5215..5432	+	218	Protein_8	transposase	-
E5285_RS23635	5437..5712	-	276	WP_001403920.1	type II toxin-antitoxin system VapC family toxin	-
E5285_RS23640	5801..6031	-	231	WP_001261274.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
E5285_RS23645	6504..8768	-	2265	WP_015060475.1	UvrD-helicase domain-containing protein	-
E5285_RS23650	8770..9546	-	777	WP_039052454.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..131158	131158
	-	flank	IS/Tn	-	-	3459..3962	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (92-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 177 a.a.
• Molecular weight: 20321.20 Da
• Isoelectric Point: 6.4889

>T123277 WP_000348883.1 NZ_CP038858:4684-5214 [Escherichia coli]
MDGLRIEIFSEEVEYQLSNFDCGEEYLNTFLTDHLQRQHSSKILRGYLLVTREDRPRVMGYYTLSGSCFEKILLPSKTQQ
KRVPYKNVPSVTLGRLAIDKSIHHQGYGETLVTHAMKVVYQASQAVGIHGMFVEALNDNAKKFYLRLGFIQLKEENCDSL
FYPTKSIEVLFEVNDE

Nucleotide

• Length: 531 bp

>T123277 NZ_CP038858:4684-5214 [Escherichia coli]
ATGGACGGTTTAAGGATTGAGATTTTTTCAGAGGAAGTTGAATATCAATTAAGCAACTTCGATTGTGGAGAAGAGTATCT
GAATACTTTTCTCACCGATCATCTGCAACGTCAGCACAGTAGTAAGATTTTACGGGGTTATCTGCTCGTGACGAGAGAGG
ATAGACCACGTGTCATGGGGTACTACACCCTGTCAGGCAGTTGCTTTGAGAAAATACTTCTCCCCAGCAAAACACAACAA
AAGCGTGTGCCCTACAAAAATGTGCCGAGCGTGACTTTGGGCCGTCTGGCAATTGATAAAAGCATTCATCACCAGGGATA
TGGAGAAACACTTGTTACCCATGCGATGAAAGTCGTGTATCAGGCCTCGCAGGCTGTGGGTATACACGGAATGTTCGTGG
AGGCGCTGAACGATAACGCCAAAAAATTCTACCTTCGACTGGGTTTTATACAGTTAAAGGAAGAAAACTGTGATTCGCTC
TTCTACCCGACAAAATCTATTGAAGTATTGTTCGAAGTAAATGACGAATAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10229.63 Da
• Isoelectric Point: 6.4702

>AT123277 WP_001275013.1 NZ_CP038858:4411-4680 [Escherichia coli]
MSALKKQRIDLRLNEDDKHMIEEAAAMTNQSISQFMVSTASARAAEVIDQHRRLLLNEESWNLVMDAITNPPAPNDRLKR
ATNRLRELE

Nucleotide

• Length: 270 bp

>AT123277 NZ_CP038858:4411-4680 [Escherichia coli]
ATGTCAGCACTGAAGAAGCAGCGCATCGATCTGAGATTAAATGAAGACGACAAACACATGATCGAAGAAGCTGCAGCAAT
GACTAACCAGTCCATTTCGCAGTTCATGGTCAGTACCGCCTCAGCACGTGCTGCTGAAGTTATTGATCAGCACCGTCGCC
TGCTCCTCAATGAGGAGTCCTGGAATCTGGTTATGGATGCAATCACTAATCCGCCAGCACCAAATGACCGGCTGAAACGC
GCCACTAACCGTCTCCGGGAACTGGAGTAA

Structures

Toxin

• AlphaFold DB: G3CAI8

Antitoxin

• AlphaFold DB: G3CAI7