Detailed information of TA system TA123275

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yfjZ-ypjF/CbtA-CbeA
• Location: 4128735..4129438
• Replicon: chromosome
• Accession: NZ_CP038857
• Organism: Escherichia coli strain PigCaeca_2

Toxin (Protein)

• Gene name: ypjF
• Uniprot ID: A0A8T6QFJ5
• Locus tag: E5285_RS20115
• Protein ID: WP_024360470.1
• Coordinates: 4128735..4129076 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: E5285_RS20120
• Protein ID: WP_096836435.1
• Coordinates: 4129097..4129438 (-)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5285_RS20105	4123946..4127062	+	3117	WP_021571035.1	HsdR family type I site-specific deoxyribonuclease	-
E5285_RS20110	4127469..4128476	-	1008	WP_135927393.1	restriction endonuclease	-
E5285_RS20115	4128735..4129076	-	342	WP_024360470.1	TA system toxin CbtA family protein	Toxin
E5285_RS20120	4129097..4129438	-	342	WP_096836435.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
E5285_RS20125	4129449..4129991	-	543	WP_017880113.1	DNA repair protein RadC	-
E5285_RS20130	4130004..4130444	-	441	WP_044060602.1	antirestriction protein	-
E5285_RS20135	4130475..4131296	-	822	WP_060175536.1	DUF945 domain-containing protein	-
E5285_RS20140	4131395..4131616	-	222	WP_094917785.1	DUF905 domain-containing protein	-
E5285_RS20145	4131688..4132137	-	450	WP_135927395.1	hypothetical protein	-
E5285_RS20150	4132134..4132586	-	453	WP_048236233.1	hypothetical protein	-
E5285_RS20155	4132623..4133192	-	570	WP_004929046.1	hypothetical protein	-
E5285_RS20160	4133192..4133896	-	705	WP_135927397.1	WYL domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-109)

Antitoxin

• Antitoxin CbeA (13-111)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12793.70 Da
• Isoelectric Point: 8.4941

>T123275 WP_024360470.1 NZ_CP038857:c4129076-4128735 [Escherichia coli]
MKTLPATNPQAATLYLSPVAVWQMLLARLLEQHYGLTLNDTPFSDETVIQEHIDAGISLADAVNFLVEKYGLVRIDRRGF
DWQEQSPYLRAVDILRARQATGLLKRNRISAAR

Nucleotide

• Length: 342 bp

>T123275 NZ_CP038857:c4129076-4128735 [Escherichia coli]
ATGAAAACTCTACCTGCAACAAATCCGCAGGCGGCGACGCTATATCTGTCGCCTGTGGCAGTCTGGCAAATGCTGCTTGC
ACGCCTGCTGGAACAACATTATGGCCTGACTCTCAACGACACACCATTCAGTGATGAAACGGTAATTCAGGAACATATCG
ATGCCGGTATATCTTTGGCCGATGCTGTTAATTTTCTGGTGGAAAAGTACGGGCTGGTTCGTATTGATCGCAGGGGATTC
GACTGGCAAGAACAGTCCCCATATCTGCGGGCAGTCGATATTCTGCGGGCGCGACAGGCAACTGGTTTATTGAAAAGAAA
TCGTATTAGCGCGGCACGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12835.52 Da
• Isoelectric Point: 6.6223

>AT123275 WP_096836435.1 NZ_CP038857:c4129438-4129097 [Escherichia coli]
MKSTDSEKDSFLKWGLNCNMTPCFGARLVQEGNRLHYLADRACITGEFSNAESLKLDEVFTRFIRQMESMLTTGEMNPRH
AHSVTLYHNGFTCEADTLGSCGYVYIAVYPTHR

Nucleotide

• Length: 342 bp

>AT123275 NZ_CP038857:c4129438-4129097 [Escherichia coli]
ATGAAATCTACTGACTCAGAGAAAGATTCATTTCTAAAATGGGGACTGAATTGTAATATGACACCTTGCTTTGGTGCTCG
TCTGGTACAGGAGGGAAACCGGCTGCACTATCTGGCTGATCGGGCCTGCATTACCGGTGAATTCAGTAACGCAGAATCTT
TGAAGTTGGATGAAGTATTCACACGTTTTATCCGCCAAATGGAATCGATGCTGACCACTGGTGAAATGAATCCTCGCCAT
GCCCACAGTGTCACCCTGTACCACAACGGTTTCACCTGCGAAGCCGATACCCTTGGCAGTTGCGGCTACGTATACATCGC
TGTTTACCCCACTCATCGTTAA