Detailed information of TA system TA123252

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Hha-TomB/-
• Location: 3618888..3619508
• Replicon: chromosome
• Accession: NZ_CP038856
• Organism: Citrobacter freundii strain Upstream_1

Toxin (Protein)

• Gene name: Hha
• Uniprot ID: R8WYV2
• Locus tag: E5284_RS17380
• Protein ID: WP_002892050.1
• Coordinates: 3619290..3619508 (+)
• Length: 73 a.a.

Antitoxin (Protein)

• Gene name: TomB
• Uniprot ID: -
• Locus tag: E5284_RS17375
• Protein ID: WP_115259201.1
• Coordinates: 3618888..3619262 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5284_RS17365	3614034..3615227	+	1194	WP_096758038.1	multidrug efflux RND transporter periplasmic adaptor subunit AcrA	-
E5284_RS17370	3615250..3618399	+	3150	WP_096758039.1	efflux RND transporter permease AcrB	-
E5284_RS17375	3618888..3619262	+	375	WP_115259201.1	Hha toxicity modulator TomB	Antitoxin
E5284_RS17380	3619290..3619508	+	219	WP_002892050.1	hemolysin expression modulator Hha	Toxin
E5284_RS17385	3619890..3620363	+	474	WP_096758041.1	YlaC family protein	-
E5284_RS17390	3620441..3620581	-	141	WP_005125190.1	type B 50S ribosomal protein L36	-
E5284_RS17395	3620583..3620843	-	261	WP_135912456.1	type B 50S ribosomal protein L31	-
E5284_RS17400	3621028..3622581	+	1554	WP_115259216.1	EAL domain-containing protein	-
E5284_RS17405	3622653..3623006	-	354	WP_115259217.1	DUF1428 family protein	-
E5284_RS17410	3623089..3623700	-	612	WP_135912457.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Haemolysin expression modulating, HHA (13-69)

Antitoxin

• Biofilm formation regulator YbaJ (1-116)

Sequences

Toxin

Protein

• Length: 73 a.a.
• Molecular weight: 8628.00 Da
• Isoelectric Point: 8.9008

>T123252 WP_002892050.1 NZ_CP038856:3619290-3619508 [Citrobacter freundii]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Nucleotide

• Length: 219 bp

>T123252 NZ_CP038856:3619290-3619508 [Citrobacter freundii]
ATGTCTGATAAACCATTAACTAAAACAGATTATTTGATGCGTCTTCGACGCTGCCAGACAATTGACACGCTCGAGCGCGT
TATTGAGAAAAATAAATATGAATTGTCCGACAATGAACTGGCTGTATTTTACTCAGCAGCAGATCATCGCCTTGCTGAAT
TGACGATGAATAAGCTGTACGACAAGATTCCAACTTCCGTTTGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14454.26 Da
• Isoelectric Point: 5.5653

>AT123252 WP_115259201.1 NZ_CP038856:3618888-3619262 [Citrobacter freundii]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEQSNHGWVNDPTSATSLQLNELIEHIATFALNYKIKYNEDNKLIAQIDEYL
DDTFMLFSNYGINTQDLQKWRKSGNRLFRCFVNATRANPVSLSC

Nucleotide

• Length: 375 bp

>AT123252 NZ_CP038856:3618888-3619262 [Citrobacter freundii]
ATGGACGAGTACTCACCCAAACGGCATGATATCGCGCAGCTTAAATTTCTCTGCGAAACCTTGTATCATGACTGTCTTGC
AAACCTTGAACAAAGCAACCATGGCTGGGTTAATGACCCGACTTCAGCAACCAGCCTGCAGCTCAACGAACTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATTGATGAATACCTG
GATGACACTTTTATGTTGTTCAGCAATTATGGTATTAATACGCAGGATTTGCAGAAATGGCGAAAGTCCGGAAATCGACT
GTTTCGCTGTTTTGTCAATGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A2P8K6F2