Detailed information of TA system TA123238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 92382..93121
• Replicon: plasmid pVag2
• Accession: NZ_CP038855
• Organism: Pantoea vagans strain LMG 24199

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: EGO56_RS21885
• Protein ID: WP_135911117.1
• Coordinates: 92382..92867 (-)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: EGO56_RS21890
• Protein ID: WP_135911118.1
• Coordinates: 92855..93121 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGO56_RS21865	89048..89368	+	321	WP_110332374.1	EamA family transporter	-
EGO56_RS21870	89365..89730	+	366	WP_135911114.1	4-amino-4-deoxy-L-arabinose-phospho-UDP flippase	-
EGO56_RS21875	89877..91406	+	1530	WP_135911115.1	Fic family protein	-
EGO56_RS21880	91438..92139	-	702	WP_135911116.1	haloacid dehalogenase type II	-
EGO56_RS21885	92382..92867	-	486	WP_135911117.1	GNAT family N-acetyltransferase	Toxin
EGO56_RS21890	92855..93121	-	267	WP_135911118.1	DUF1778 domain-containing protein	Antitoxin
EGO56_RS21895	93230..94036	-	807	WP_135911119.1	ABC transporter permease	-
EGO56_RS21900	94029..94895	-	867	WP_135911120.1	ABC transporter permease	-
EGO56_RS21905	94895..95977	-	1083	WP_135911121.1	ABC transporter ATP-binding protein	-
EGO56_RS21910	95974..97050	-	1077	WP_135911122.1	ABC transporter substrate-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	-	1..180464	180464

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17646.45 Da
• Isoelectric Point: 8.7625

>T123238 WP_135911117.1 NZ_CP038855:c92867-92382 [Pantoea vagans]
VGPVAAPEPLSSTHQLAEFISGEAVLDDWLKQRGLNSQALGAARTFVVCKTDTKQVAGFYSLATGSVNHLEAMRNLRRNM
PDPIPVIILARLAVDVSFRGSGLGADLLHDAVLRCCRVAENIGVRAMMVHALTEEAKRFYLHHGFKASQIQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T123238 NZ_CP038855:c92867-92382 [Pantoea vagans]
GTGGGACCTGTAGCAGCACCAGAACCGTTATCCAGTACCCATCAACTGGCTGAGTTCATCAGTGGTGAGGCTGTACTTGA
TGACTGGCTTAAACAGAGAGGCTTAAATAGCCAGGCTCTCGGTGCAGCCAGAACGTTCGTTGTTTGTAAAACAGACACAA
AACAAGTTGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTTAACCACCTGGAAGCGATGAGAAACCTCAGGCGCAATATG
CCCGATCCCATTCCCGTAATCATACTTGCTCGCCTGGCAGTCGATGTTTCATTCCGGGGCTCGGGGCTCGGGGCTGATTT
ACTTCATGATGCAGTTCTGCGTTGCTGCCGTGTAGCTGAGAATATTGGAGTCCGGGCGATGATGGTTCATGCATTGACTG
AAGAAGCCAAACGCTTTTATCTTCACCATGGATTTAAGGCATCGCAAATCCAGGAACGGACATTGTTTCTCAAGCTGCCG
CAGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9990.36 Da
• Isoelectric Point: 4.4608

>AT123238 WP_135911118.1 NZ_CP038855:c93121-92855 [Pantoea vagans]
MKSDVQLNLRAKDSQRALIDAAAEILHKSRTDFILEVACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVSDAAAIDKLL
ARKPQWDL

Nucleotide

• Length: 267 bp

>AT123238 NZ_CP038855:c93121-92855 [Pantoea vagans]
ATGAAATCAGATGTTCAGCTCAACCTCAGAGCTAAGGATTCTCAAAGAGCACTCATTGATGCTGCAGCAGAGATTCTCCA
TAAATCACGCACAGACTTCATCCTGGAAGTGGCATGCCAGGCTGCCGAGAATGTGATCCTCGATCGTCGGGTATTTAATT
TTAACGATGAGCAGTATGCCGAGTTTATCGATATGCTTGATGCACCCGTTTCGGATGCTGCTGCCATTGATAAACTCCTG
GCGAGGAAACCTCAGTGGGACCTGTAG