Detailed information of TA system TA123236

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 505922..506832
• Replicon: plasmid pVag1
• Accession: NZ_CP038854
• Organism: Pantoea vagans strain LMG 24199

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: EGO56_RS21255
• Protein ID: WP_135910988.1
• Coordinates: 506362..506832 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: D6YQ03
• Locus tag: EGO56_RS21250
• Protein ID: WP_013195990.1
• Coordinates: 505922..506365 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGO56_RS21225	501177..501902	-	726	WP_010246779.1	ABC transporter permease subunit	-
EGO56_RS21230	501909..502757	-	849	WP_013195994.1	transporter substrate-binding domain-containing protein	-
EGO56_RS21235	502779..503966	-	1188	WP_135910985.1	amidohydrolase	-
EGO56_RS21240	504241..505107	-	867	WP_135910986.1	alpha/beta hydrolase	-
EGO56_RS21245	505159..505773	-	615	WP_135910987.1	TetR/AcrR family transcriptional regulator	-
EGO56_RS21250	505922..506365	+	444	WP_013195990.1	DUF2384 domain-containing protein	Antitoxin
EGO56_RS21255	506362..506832	+	471	WP_135910988.1	RES family NAD+ phosphorylase	Toxin
EGO56_RS21260	507006..508037	+	1032	WP_135910989.1	AI-2E family transporter	-
EGO56_RS21265	508026..510755	-	2730	WP_135910990.1	cation-transporting P-type ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla / vipA/tssB / vipB/tssC / vasE/tssK / dotU/tssL / hcp/tssD / clpV/tssH / vgrG/tssI / KPHS_23120 / icmF/tssM / tssF / tssG / sciN/tssJ / iroN	1..559692	559692

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (32-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17452.87 Da
• Isoelectric Point: 4.9584

>T123236 WP_135910988.1 NZ_CP038854:506362-506832 [Pantoea vagans]
MILYRLTKTKYLSTAWTGFSAKEAGGRWNSIGISMVYVSETASLTLLETLVHLHSAHILDAFTLLRIDVPDALIQTVDIS
ELPANWADEDAPAELADYGDAWCSACDAVALRVPSALSPIEHNYLLNPEHPEFSRIVQQAEAIPFRFDRRLKPDRK

Nucleotide

• Length: 471 bp

>T123236 NZ_CP038854:506362-506832 [Pantoea vagans]
TTGATCCTCTACAGGCTGACTAAGACAAAGTATCTTTCGACCGCCTGGACGGGATTCAGCGCAAAAGAGGCGGGCGGTCG
CTGGAACAGCATCGGCATCTCAATGGTTTATGTCTCAGAAACCGCCTCACTGACGTTGCTGGAAACACTGGTCCATCTGC
ACTCCGCACACATCCTCGACGCTTTCACGCTACTGCGCATCGATGTTCCCGATGCGCTGATACAAACCGTCGATATAAGT
GAATTGCCTGCAAACTGGGCCGATGAAGACGCGCCGGCGGAACTGGCTGACTATGGCGACGCCTGGTGCTCTGCCTGCGA
TGCGGTTGCGTTGCGTGTACCCAGTGCCCTCTCGCCCATAGAACACAACTATCTGTTAAATCCTGAACACCCGGAGTTTT
CCCGGATCGTTCAACAGGCTGAGGCCATTCCGTTCCGGTTTGACCGTCGGCTGAAACCGGACCGTAAATAG

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16453.83 Da
• Isoelectric Point: 9.5911

>AT123236 WP_013195990.1 NZ_CP038854:505922-506365 [Pantoea vagans]
MKTFSFSAAKTRPPRLWQAAGLHSTDGVALLGQINDGLDGNVAHLIIDWAKITQNDLRRMSGIPSTTFSRGVKAKFSAEQ
SERLVRIIRVIDRAVELFEGDKDEAQKWLNEPNRALSWKMPAELMASETGAYEVLKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT123236 NZ_CP038854:505922-506365 [Pantoea vagans]
ATGAAAACATTCAGCTTTTCTGCAGCTAAGACCAGACCGCCACGTTTATGGCAGGCTGCGGGCCTGCACAGTACGGATGG
TGTCGCTCTGCTGGGCCAGATCAACGATGGCCTTGATGGCAACGTGGCTCACCTCATCATCGACTGGGCGAAGATTACCC
AAAACGACCTGCGCAGAATGTCCGGTATTCCCTCCACGACATTCAGCCGTGGCGTGAAAGCAAAATTCAGCGCTGAACAA
AGCGAACGACTGGTGCGAATTATCCGCGTTATTGATCGGGCGGTTGAATTGTTCGAAGGCGATAAGGATGAAGCGCAAAA
ATGGCTGAATGAGCCTAACCGGGCGTTGAGCTGGAAAATGCCAGCAGAACTTATGGCATCGGAAACTGGCGCTTATGAGG
TCCTGAAGCTCATCACGCGTCTGGAGCATGGAGTTTATTCTTGA

Structures

Antitoxin

• AlphaFold DB: A0A7Z1Y7E4