123232

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3049902..3050519 Replicon chromosome
Accession NZ_CP038853
Organism Pantoea vagans strain LMG 24199

Toxin (Protein)


Gene name Hha Uniprot ID E0LTU7
Locus tag EGO56_RS14475 Protein ID WP_003850458.1
Coordinates 3050304..3050519 (+) Length 72 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID E1SGC9
Locus tag EGO56_RS14470 Protein ID WP_013357010.1
Coordinates 3049902..3050279 (+) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EGO56_RS14440 3046217..3046471 + 255 WP_033782196.1 type B 50S ribosomal protein L31 -
EGO56_RS14445 3046483..3046623 + 141 WP_009091664.1 type B 50S ribosomal protein L36 -
EGO56_RS14450 3046669..3047547 - 879 WP_135909859.1 metal ABC transporter substrate-binding protein -
EGO56_RS14455 3047567..3048406 - 840 WP_135909861.1 metal ABC transporter permease -
EGO56_RS14460 3048403..3049071 - 669 WP_135909863.1 ABC transporter ATP-binding protein -
EGO56_RS14465 3049401..3049754 + 354 WP_135910588.1 hypothetical protein -
EGO56_RS14470 3049902..3050279 + 378 WP_013357010.1 Hha toxicity modulator TomB Antitoxin
EGO56_RS14475 3050304..3050519 + 216 WP_003850458.1 hemolysin expression modulator Hha Toxin
EGO56_RS14485 3050942..3051286 + 345 WP_135909864.1 MGMT family protein -
EGO56_RS14490 3051288..3051845 - 558 WP_033731772.1 YbaY family lipoprotein -
EGO56_RS14495 3052036..3052899 + 864 WP_013357007.1 acyl-CoA thioesterase II -
EGO56_RS14500 3052958..3054244 - 1287 WP_013357006.1 ammonium transporter AmtB -
EGO56_RS14505 3054279..3054617 - 339 WP_003850469.1 P-II family nitrogen regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(12-68)

Antitoxin

Biofilm formation regulator YbaJ

(1-114)


Sequences

Toxin        


Download         Length: 72 a.a.        Molecular weight: 8510.90 Da        Isoelectric Point: 9.4825

>T123232 WP_003850458.1 NZ_CP038853:3050304-3050519 [Pantoea vagans]
MNKTLTKTDYLMRLRRCRSLDTLERVIEKNKYELPEDELAVFYSAADHRLAELTMNKLYDKVPGSVWKFVR

Download         Length: 216 bp

>T123232 NZ_CP038853:3050304-3050519 [Pantoea vagans]
ATGAACAAAACCCTGACTAAAACCGATTATTTGATGCGACTGCGGCGTTGCCGCTCTCTGGATACCCTGGAAAGGGTCAT
TGAAAAAAATAAGTATGAATTACCCGAGGATGAATTAGCGGTATTTTACTCTGCAGCTGACCATCGTCTGGCGGAACTGA
CGATGAACAAGCTCTACGATAAAGTCCCAGGTTCAGTATGGAAATTTGTCCGTTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 14650.28 Da        Isoelectric Point: 4.3976

>AT123232 WP_013357010.1 NZ_CP038853:3049902-3050279 [Pantoea vagans]
MDEYSPKRHDIAQLKYLCESLFDDSMATLTDSHHGWVNDPTSESNLQLNDLIEHIASFTMNYKIKHVEDEALISQIDEYL
DDTFMLFSNYGINSPDLQRWQRSAKRLFNLFTEECAFLQQPSHSL

Download         Length: 378 bp

>AT123232 NZ_CP038853:3049902-3050279 [Pantoea vagans]
ATGGACGAATACTCACCGAAACGGCATGATATTGCCCAGCTTAAATATCTGTGTGAGAGCCTGTTTGACGACAGCATGGC
AACATTAACCGACAGCCATCACGGCTGGGTGAATGACCCCACTTCTGAAAGCAATCTGCAGCTTAACGATTTGATTGAAC
ACATTGCCTCTTTCACCATGAATTACAAAATTAAGCACGTTGAAGATGAAGCGCTGATTTCGCAAATCGATGAATATCTT
GATGATACTTTTATGCTATTCAGCAACTATGGTATCAATAGTCCGGATCTTCAACGGTGGCAACGTTCAGCAAAACGCTT
ATTTAATCTGTTCACTGAAGAGTGCGCTTTTCTCCAGCAACCAAGCCATTCATTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1I5AGC3

Antitoxin

Source ID Structure
AlphaFold DB A0A5M7PH97

References