Detailed information of TA system TA123231

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicB(antitoxin)
• Location: 2166871..2167501
• Replicon: chromosome
• Accession: NZ_CP038853
• Organism: Pantoea vagans strain LMG 24199

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: EGO56_RS10095
• Protein ID: WP_135908896.1
• Coordinates: 2167319..2167501 (-)
• Length: 61 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: EGO56_RS10090
• Protein ID: WP_135908894.1
• Coordinates: 2166871..2167296 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGO56_RS10075	2162093..2163247	+	1155	WP_033732608.1	efflux RND transporter periplasmic adaptor subunit	-
EGO56_RS10080	2163260..2166412	+	3153	WP_135908892.1	multidrug efflux RND transporter permease subunit OqxB	-
EGO56_RS10085	2166482..2166836	-	355	Protein_1949	type II toxin-antitoxin system HicB family antitoxin	-
EGO56_RS10090	2166871..2167296	-	426	WP_135908894.1	hypothetical protein	Antitoxin
EGO56_RS10095	2167319..2167501	-	183	WP_135908896.1	type II toxin-antitoxin system HicA family toxin	Toxin
EGO56_RS10100	2167696..2168190	-	495	WP_013357792.1	Rrf2 family transcriptional regulator	-
EGO56_RS10105	2168404..2169603	-	1200	WP_167493431.1	MFS transporter	-
EGO56_RS10110	2169790..2170719	+	930	WP_135908900.1	DNA replication terminus site-binding protein	-
EGO56_RS10115	2170761..2172158	-	1398	WP_033783020.1	class II fumarate hydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (3-60)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (4-82)

Sequences

Toxin

Protein

• Length: 61 a.a.
• Molecular weight: 7034.14 Da
• Isoelectric Point: 11.0643

>T123231 WP_135908896.1 NZ_CP038853:c2167501-2167319 [Pantoea vagans]
MKYSEFRRWLIQQGAIFTKHRSGSSHFKVFLGDRQSIFPDHGAKEMPEGTRRKILKDLGL

Nucleotide

• Length: 183 bp

>T123231 NZ_CP038853:c2167501-2167319 [Pantoea vagans]
ATGAAGTACAGTGAATTCAGACGCTGGCTCATTCAGCAGGGCGCTATTTTTACTAAGCACCGCAGTGGAAGCAGCCATTT
CAAGGTCTTCCTGGGCGACAGGCAATCCATTTTTCCGGATCATGGAGCGAAAGAAATGCCTGAAGGTACGCGCAGAAAAA
TTTTAAAAGATCTGGGCCTGTGA

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15462.78 Da
• Isoelectric Point: 4.9966

>AT123231 WP_135908894.1 NZ_CP038853:c2167296-2166871 [Pantoea vagans]
MFNYAVSLTHDARTDTYEVSCRDLPLLHSVGATVEEALSEAEDGLVTAISLEMDTRRPIPVASKPLEGEYIVSVPVLVAM
KAALYNAMIETGTRKVDLARQLGVNGPQVDRLLDVEHSSKVEKVEMALAQLGRKARIEIRT

Nucleotide

• Length: 426 bp

>AT123231 NZ_CP038853:c2167296-2166871 [Pantoea vagans]
ATGTTTAATTATGCCGTCAGCTTAACCCATGACGCCAGAACCGATACCTATGAGGTCTCGTGTCGTGATTTGCCGTTACT
GCATTCCGTCGGCGCCACCGTTGAGGAAGCACTGTCAGAAGCCGAAGATGGACTGGTGACGGCTATCTCTCTTGAGATGG
ATACCCGTCGTCCGATTCCGGTCGCCAGTAAACCGCTGGAAGGTGAATACATTGTCTCCGTACCTGTGTTGGTGGCGATG
AAAGCGGCACTTTATAACGCAATGATTGAAACCGGCACTCGTAAAGTCGATCTGGCACGCCAGCTGGGCGTGAATGGCCC
ACAGGTTGATCGACTGCTGGATGTTGAGCACTCATCGAAAGTTGAAAAAGTGGAGATGGCGCTGGCGCAACTGGGACGAA
AAGCCCGAATTGAGATTCGCACCTGA