Detailed information of TA system TA123228

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 1486799..1487389
• Replicon: chromosome
• Accession: NZ_CP038853
• Organism: Pantoea vagans strain LMG 24199

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: -
• Locus tag: EGO56_RS06905
• Protein ID: WP_135908176.1
• Coordinates: 1487057..1487389 (+)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: -
• Locus tag: EGO56_RS06900
• Protein ID: WP_135910538.1
• Coordinates: 1486799..1487056 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
EGO56_RS06890	1482782..1483804	+	1023	WP_135908175.1	LLM class flavin-dependent oxidoreductase	-
EGO56_RS06895	1484292..1486469	+	2178	WP_135910537.1	catalase/peroxidase HPI	-
EGO56_RS06900	1486799..1487056	+	258	WP_135910538.1	antitoxin	Antitoxin
EGO56_RS06905	1487057..1487389	+	333	WP_135908176.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
EGO56_RS06910	1487495..1488565	-	1071	WP_135908177.1	ATP-binding cassette domain-containing protein	-
EGO56_RS06915	1488562..1489542	-	981	WP_135908178.1	ABC transporter ATP-binding protein	-
EGO56_RS06920	1489539..1490486	-	948	WP_167493445.1	ABC transporter permease	-
EGO56_RS06925	1490486..1491445	-	960	WP_135908180.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-108)

Antitoxin

• Antitoxin PemI-like (5-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11998.78 Da
• Isoelectric Point: 5.8600

>T123228 WP_135908176.1 NZ_CP038853:1487057-1487389 [Pantoea vagans]
MERGEIWLVTLDPTAGHEQSGKRPVLIVSPASFNAFTRLPIVVPVTSGGNFARSAGFVVSLDEAGCRTKGVIRCDQPRTL
DMEAREGRRLEQLPDAIVNEVLARLEAILN

Nucleotide

• Length: 333 bp

>T123228 NZ_CP038853:1487057-1487389 [Pantoea vagans]
ATGGAGAGAGGGGAAATCTGGCTGGTGACACTCGATCCCACCGCAGGTCATGAACAAAGTGGAAAACGTCCGGTACTGAT
CGTTTCTCCGGCCTCGTTTAACGCGTTCACCCGATTACCGATTGTGGTGCCGGTCACCAGCGGCGGAAATTTTGCCCGCT
CAGCAGGTTTTGTGGTTTCACTGGATGAGGCGGGTTGCAGGACAAAGGGCGTCATTCGCTGCGATCAGCCGAGGACGCTC
GATATGGAAGCCCGCGAAGGCAGGCGGCTTGAACAACTACCTGACGCTATCGTGAATGAAGTGCTGGCACGTCTTGAAGC
CATTCTGAACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9339.83 Da
• Isoelectric Point: 6.9757

>AT123228 WP_135910538.1 NZ_CP038853:1486799-1487056 [Pantoea vagans]
MFITRLKKVGGSVMLAVPPAVLKTLALSTNSEVGMTIDNGYLIVKPQNRPRYSLEELLAQCDPHAQMSTEDREWINAPAA
GKENL

Nucleotide

• Length: 258 bp

>AT123228 NZ_CP038853:1486799-1487056 [Pantoea vagans]
ATGTTTATCACGCGTCTTAAAAAGGTTGGCGGATCGGTCATGCTGGCGGTTCCTCCCGCCGTTCTGAAAACCCTGGCATT
GTCGACAAACAGCGAAGTCGGTATGACTATCGATAATGGTTATCTGATCGTTAAGCCCCAGAATCGCCCGCGATATTCAC
TCGAGGAACTGCTGGCGCAATGCGATCCACACGCTCAAATGAGCACAGAGGATCGGGAATGGATTAATGCGCCTGCAGCA
GGTAAGGAGAACCTCTAA