Detailed information of TA system TA123221

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2087591..2088120
• Replicon: chromosome
• Accession: NZ_CP038819
• Organism: Staphylococcus aureus strain O82

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SaO82_RS10580
• Protein ID: WP_000621175.1
• Coordinates: 2087591..2087953 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SaO82_RS10585
• Protein ID: WP_000948331.1
• Coordinates: 2087950..2088120 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO82_RS10555	2084569..2085339	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SaO82_RS10560	2085314..2085793	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SaO82_RS10565	2085795..2086121	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SaO82_RS10570	2086240..2087241	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SaO82_RS10580	2087591..2087953	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SaO82_RS10585	2087950..2088120	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SaO82_RS10590	2088205..2089353	-	1149	WP_001281143.1	alanine racemase	-
SaO82_RS10595	2089419..2089778	-	360	WP_000581198.1	holo-ACP synthase	-
SaO82_RS10600	2089782..2090273	-	492	WP_001205919.1	PH domain-containing protein	-
SaO82_RS10605	2090260..2091843	-	1584	WP_001294641.1	PH domain-containing protein	-
SaO82_RS10610	2091836..2092315	-	480	WP_001287083.1	hypothetical protein	-
SaO82_RS10615	2092524..2093084	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T123221 WP_000621175.1 NZ_CP038819:c2087953-2087591 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T123221 NZ_CP038819:c2087953-2087591 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT123221 WP_000948331.1 NZ_CP038819:c2088120-2087950 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT123221 NZ_CP038819:c2088120-2087950 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1