Detailed information of TA system TA123212

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/YoeB-YefM
• Location: 1804338..1804840
• Replicon: chromosome
• Accession: NZ_CP038817
• Organism: Haemophilus parahaemolyticus strain NEB129

Toxin (Protein)

• Gene name: relE
• Uniprot ID: I3D875
• Locus tag: E5Q53_RS08710
• Protein ID: WP_005707339.1
• Coordinates: 1804338..1804592 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: I3D869
• Locus tag: E5Q53_RS08715
• Protein ID: WP_005707332.1
• Coordinates: 1804589..1804840 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Q53_RS08690	1800045..1800512	-	468	WP_005707325.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
E5Q53_RS08695	1800704..1802083	+	1380	WP_005707288.1	DNA repair protein RadA	-
E5Q53_RS08700	1802098..1802988	+	891	WP_039863409.1	RimK family alpha-L-glutamate ligase	-
E5Q53_RS08705	1802990..1804324	-	1335	WP_039863408.1	23S rRNA (uracil(1939)-C(5))-methyltransferase RlmD	-
E5Q53_RS08710	1804338..1804592	-	255	WP_005707339.1	Txe/YoeB family addiction module toxin	Toxin
E5Q53_RS08715	1804589..1804840	-	252	WP_005707332.1	YoeB-YefM toxin-antitoxin system antitoxin YefM	Antitoxin
E5Q53_RS08720	1804991..1807899	+	2909	Protein_1660	RNA polymerase-associated protein RapA	-
E5Q53_RS08725	1807902..1808570	+	669	WP_005707293.1	bifunctional tRNA pseudouridine(32) synthase/23S rRNA pseudouridine(746) synthase RluA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-71)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 10067.52 Da
• Isoelectric Point: 9.3942

>T123212 WP_005707339.1 NZ_CP038817:c1804592-1804338 [Haemophilus parahaemolyticus]
MKLTFADTAWEDYLYWQATDKKQLKRTNNLIKDIQRTPFEGIGKPESLKFHLSGFWSRRIDEKHRLVYAVTDEAILIASC
RYHY

Nucleotide

• Length: 255 bp

>T123212 NZ_CP038817:c1804592-1804338 [Haemophilus parahaemolyticus]
ATGAAATTGACCTTTGCCGACACCGCTTGGGAAGATTATTTATATTGGCAAGCCACCGACAAAAAACAACTAAAACGCAC
CAATAATCTCATCAAGGATATTCAACGAACGCCTTTTGAAGGTATAGGAAAACCTGAATCATTGAAGTTTCATTTAAGTG
GTTTTTGGTCTCGTCGAATTGATGAAAAACACAGGCTGGTTTATGCCGTAACTGATGAAGCAATTTTAATTGCTTCTTGT
CGTTATCATTACTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9403.58 Da
• Isoelectric Point: 4.7411

>AT123212 WP_005707332.1 NZ_CP038817:c1804840-1804589 [Haemophilus parahaemolyticus]
MQAISYSQARQTLAKTMTSVVENYEPVLITRTTGEDCVLISLAHYRSLEETAYLLRSPANAQHLLSAIEQLHQNEGQERE
LIE

Nucleotide

• Length: 252 bp

>AT123212 NZ_CP038817:c1804840-1804589 [Haemophilus parahaemolyticus]
ATGCAAGCTATTTCTTATTCACAAGCCCGTCAAACTTTAGCCAAAACAATGACTTCAGTCGTGGAAAATTATGAACCTGT
GCTAATCACTCGCACTACGGGCGAAGATTGTGTGCTTATTTCTCTAGCACATTATCGTTCACTAGAGGAAACTGCTTATT
TGCTTCGTTCGCCTGCCAATGCTCAACACTTACTTTCAGCCATTGAGCAACTTCACCAAAATGAAGGGCAAGAGAGGGAA
TTGATTGAATGA

Structures

Toxin

• AlphaFold DB: I3D875

Antitoxin

• AlphaFold DB: I3D869