Detailed information of TA system TA123204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 36446..37089
• Replicon: plasmid unnamed2
• Accession: NZ_CP038813
• Organism: Piscirickettsia salmonis strain Psal-001

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: E9LHC6
• Locus tag: Psal001_RS17535
• Protein ID: WP_016210974.1
• Coordinates: 36682..37089 (+)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: E9LHC5
• Locus tag: Psal001_RS17530
• Protein ID: WP_016210972.1
• Coordinates: 36446..36673 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Psal001_RS17475	31543..31848	+	306	WP_016210958.1	hypothetical protein	-
Psal001_RS17480	32002..32301	+	300	WP_016210976.1	hypothetical protein	-
Psal001_RS17485	32348..33163	+	816	WP_016210962.1	hypothetical protein	-
Psal001_RS17490	33186..33746	+	561	WP_016210973.1	hypothetical protein	-
Psal001_RS17495	33715..34077	+	363	WP_016210959.1	hypothetical protein	-
Psal001_RS17500	34070..34417	+	348	WP_016210966.1	hypothetical protein	-
Psal001_RS17505	34414..34653	+	240	WP_016210963.1	hypothetical protein	-
Psal001_RS17510	34646..35035	+	390	WP_155046772.1	hypothetical protein	-
Psal001_RS17515	35032..35316	+	285	WP_036794070.1	hypothetical protein	-
Psal001_RS17520	35318..35791	+	474	WP_016210960.1	single-stranded DNA-binding protein	-
Psal001_RS17525	35906..36298	+	393	WP_129556726.1	hypothetical protein	-
Psal001_RS17530	36446..36673	+	228	WP_016210972.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
Psal001_RS17535	36682..37089	+	408	WP_016210974.1	DUF4411 family protein	Toxin
Psal001_RS17540	37206..37886	+	681	WP_129556725.1	DNA/RNA non-specific endonuclease	-
Psal001_RS17545	38065..38358	+	294	WP_016210977.1	zinc ribbon domain-containing protein	-
Psal001_RS17550	38576..38758	-	183	WP_129556724.1	hypothetical protein	-
Psal001_RS17555	38903..39082	-	180	WP_155046773.1	phosphatase	-
Psal001_RS17560	39177..39494	+	318	WP_129556723.1	hypothetical protein	-
Psal001_RS17565	39795..40178	+	384	WP_032126134.1	HNH endonuclease	-
Psal001_RS17570	40266..40745	+	480	WP_016212234.1	P27 family phage terminase small subunit	-
Psal001_RS17575	40749..40958	+	210	WP_016212231.1	hypothetical protein	-
Psal001_RS17580	41013..41330	+	318	WP_164997282.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..79942	79942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-126)

Antitoxin

• SpoVT-AbrB domain (7-41)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 15625.00 Da
• Isoelectric Point: 7.4170

>T123204 WP_016210974.1 NZ_CP038813:36682-37089 [Piscirickettsia salmonis]
MLYMLDTNICIYLIKQKPRSYYERLMEVEKSRHILAISSIVLSELQFGVARSQHQRKNQEAINTLVNKLEVLPYEEKAAK
YYGDLRADLQKKGTVIGGNDMIIAAHALSEKAVLVTHNTKEFQRVEHLEFEDWAS

Nucleotide

• Length: 408 bp

>T123204 NZ_CP038813:36682-37089 [Piscirickettsia salmonis]
ATGCTTTATATGCTGGATACGAATATTTGTATTTATTTAATTAAACAAAAGCCTCGTTCTTACTATGAGCGTTTGATGGA
GGTAGAAAAATCTCGTCATATATTGGCGATTTCATCCATTGTTTTATCTGAATTACAGTTTGGAGTGGCTCGGAGTCAAC
ACCAGCGAAAAAACCAGGAAGCGATTAATACCTTGGTAAATAAGCTAGAAGTTTTACCCTATGAGGAAAAGGCAGCTAAA
TACTATGGTGACTTAAGGGCTGACTTACAAAAAAAAGGGACTGTGATTGGTGGGAATGATATGATCATAGCCGCGCATGC
GCTCTCAGAAAAAGCCGTTTTAGTGACACATAATACAAAAGAGTTTCAGCGTGTCGAGCATTTAGAGTTTGAAGACTGGG
CAAGTTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8929.02 Da
• Isoelectric Point: 4.5679

>AT123204 WP_016210972.1 NZ_CP038813:36446-36673 [Piscirickettsia salmonis]
MAKSRIFKSGNSQAVRLPKEMRLDGEEVVIHRFGNSLLIQPVYETWMDVYNHMPDVSDDFMEDHQDIQAQERDWF

Nucleotide

• Length: 228 bp

>AT123204 NZ_CP038813:36446-36673 [Piscirickettsia salmonis]
ATGGCAAAATCACGAATTTTTAAAAGTGGTAATTCCCAAGCCGTTCGGTTACCGAAAGAAATGCGGCTGGATGGTGAAGA
AGTGGTTATTCATCGGTTTGGCAACTCTTTATTAATCCAGCCAGTTTATGAAACCTGGATGGATGTTTACAATCATATGC
CGGATGTATCGGATGATTTTATGGAAGATCATCAAGATATACAAGCACAAGAACGTGACTGGTTTTAG

Structures

Toxin

• AlphaFold DB: E9LHC6

Antitoxin

• AlphaFold DB: E9LHC5