Detailed information of TA system TA123188

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 464735..465272
• Replicon: chromosome
• Accession: NZ_CP038806
• Organism: Burkholderia pseudomallei strain HNBP001

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: Q2T6G2
• Locus tag: E5Y92_RS22260
• Protein ID: WP_004549731.1
• Coordinates: 465003..465272 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: E5Y92_RS22255
• Protein ID: WP_004525420.1
• Coordinates: 464735..465019 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E5Y92_RS22220	459843..461105	-	1263	WP_004551329.1	c-type cytochrome	-
E5Y92_RS22225	461264..462724	-	1461	WP_011326543.1	metallophosphoesterase	-
E5Y92_RS22230	462971..463087	+	117	Protein_364	IS3 family transposase	-
E5Y92_RS22235	463314..463742	-	429	Protein_365	IS110 family transposase	-
E5Y92_RS22240	463705..464088	-	384	Protein_366	DDE-type integrase/transposase/recombinase	-
E5Y92_RS22255	464735..465019	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
E5Y92_RS22260	465003..465272	-	270	WP_004549731.1	BrnT family toxin	Toxin
E5Y92_RS22265	465843..466118	+	276	WP_038716556.1	hypothetical protein	-
E5Y92_RS22270	466128..466259	+	132	WP_009923452.1	bacteriophage protein Gp48	-
E5Y92_RS22280	466397..466639	+	243	WP_004545584.1	hypothetical protein	-
E5Y92_RS37715	466636..466794	+	159	WP_004549802.1	hypothetical protein	-
E5Y92_RS37720	466842..467060	+	219	WP_004552392.1	hypothetical protein	-
E5Y92_RS22290	467291..467872	-	582	WP_004542548.1	hypothetical protein	-
E5Y92_RS22295	468128..468277	+	150	WP_004542626.1	bacteriophage protein Gp44	-
E5Y92_RS38280	468274..469047	+	774	Protein_376	prohibitin family protein	-
E5Y92_RS38285	469045..469473	+	429	Protein_377	DDE-type integrase/transposase/recombinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	457615..514680	57065

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (5-81)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10157.46 Da
• Isoelectric Point: 5.6831

>T123188 WP_004549731.1 NZ_CP038806:c465272-465003 [Burkholderia pseudomallei]
MDITFDPTKNETNIAKHGVSLALAAQLDWSDVLSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKR
EVKSYVEQA

Nucleotide

• Length: 270 bp

>T123188 NZ_CP038806:c465272-465003 [Burkholderia pseudomallei]
ATGGACATCACCTTTGACCCGACCAAGAACGAAACGAACATTGCCAAGCATGGAGTGTCGTTGGCGCTTGCAGCGCAACT
CGACTGGTCGGACGTGTTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCG
GCGATCGCCTCTATTGCGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGG
GAGGTCAAGAGCTATGTCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT123188 WP_004525420.1 NZ_CP038806:c465019-464735 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT123188 NZ_CP038806:c465019-464735 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGTCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Toxin

• AlphaFold DB: Q2T6G2

Antitoxin

• AlphaFold DB: A0A0F6L9S8