Detailed information of TA system TA123185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 842489..843054
• Replicon: chromosome
• Accession: NZ_CP038804
• Organism: Treponema putidum strain OMZ 835

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E4N74_RS03975
• Protein ID: WP_255806270.1
• Coordinates: 842716..843054 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: E4N74_RS03970
• Protein ID: WP_255818995.1
• Coordinates: 842489..842722 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4N74_RS03950 (E4N74_04070)	837775..838029	+	255	WP_044978220.1	hypothetical protein	-
E4N74_RS03955 (E4N74_04075)	838622..840229	+	1608	WP_255818992.1	hypothetical protein	-
E4N74_RS03960 (E4N74_04085)	840491..840901	+	411	WP_255818993.1	TerB family tellurite resistance protein	-
E4N74_RS03965 (E4N74_04090)	840901..841971	+	1071	WP_255818994.1	hypothetical protein	-
E4N74_RS03970 (E4N74_04095)	842489..842722	+	234	WP_255818995.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4N74_RS03975 (E4N74_04100)	842716..843054	+	339	WP_255806270.1	endoribonuclease MazF	Toxin
E4N74_RS03980 (E4N74_04105)	843215..843913	+	699	WP_010690396.1	hypothetical protein	-
E4N74_RS03985 (E4N74_04110)	844302..844874	+	573	WP_049992339.1	hypothetical protein	-
E4N74_RS03990 (E4N74_04115)	845309..846151	+	843	WP_255818997.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	835946..844874	8928
	-	inside	Integron	-	-	834481..844082	9601

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• SpoVT-AbrB domain (6-45)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12866.70 Da
• Isoelectric Point: 6.2183

>T123185 WP_255806270.1 NZ_CP038804:842716-843054 [Treponema putidum]
MVNSNYVPDRGDLVWLDFTPQEGHEQKGRRPAICISQKRYNQKVGLALFCPITSHIKGYPFEIVLDNHSINGCILSDQVK
NLDYKKRSCDFIEKTTEEEINAVVDNIKLLIE

Nucleotide

• Length: 339 bp

>T123185 NZ_CP038804:842716-843054 [Treponema putidum]
ATGGTGAATTCAAATTATGTTCCCGATAGAGGCGATTTAGTTTGGCTTGATTTTACCCCGCAAGAAGGACATGAGCAGAA
AGGTCGTCGTCCTGCAATTTGCATTTCTCAAAAACGATACAATCAAAAAGTCGGACTTGCTTTATTTTGTCCTATTACAA
GCCATATAAAAGGCTATCCTTTTGAAATTGTATTGGATAATCATTCGATAAATGGCTGTATTCTTAGCGACCAAGTAAAG
AACCTTGATTATAAAAAAAGAAGTTGTGATTTTATAGAAAAAACAACAGAAGAAGAGATAAATGCTGTTGTAGATAATAT
AAAGTTGTTGATTGAGTAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8602.88 Da
• Isoelectric Point: 5.7928

>AT123185 WP_255818995.1 NZ_CP038804:842489-842722 [Treponema putidum]
MQVVVQKWGNSLGFRIPSLWAKDNNVKNGSKIEVIAEKGKIVILPQEKTLDDMLAMVTSEKVHSEISTGYSVGNEEW

Nucleotide

• Length: 234 bp

>AT123185 NZ_CP038804:842489-842722 [Treponema putidum]
ATGCAGGTTGTAGTTCAGAAATGGGGTAATAGTCTCGGGTTTAGAATACCCTCTCTTTGGGCAAAAGATAATAATGTAAA
GAATGGAAGCAAAATAGAAGTAATCGCAGAAAAAGGAAAAATAGTAATTCTTCCTCAGGAAAAAACCCTTGATGATATGC
TGGCAATGGTTACTTCTGAAAAAGTTCATTCGGAAATCTCTACAGGTTATTCAGTAGGTAACGAAGAATGGTGA