Detailed information of TA system TA123181

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 913128..913693
• Replicon: chromosome
• Accession: NZ_CP038803
• Organism: Treponema putidum strain OMZ 846

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E4N75_RS04395
• Protein ID: WP_255806270.1
• Coordinates: 913355..913693 (+)
• Length: 113 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A562SC65
• Locus tag: E4N75_RS04390
• Protein ID: WP_044979179.1
• Coordinates: 913128..913361 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4N75_RS04365 (E4N75_04370)	908352..908831	+	480	WP_255824080.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4N75_RS04370	908836..909003	+	168	WP_255824081.1	helix-turn-helix transcriptional regulator	-
E4N75_RS04375 (E4N75_04380)	909104..910729	+	1626	WP_255824083.1	hypothetical protein	-
E4N75_RS04380 (E4N75_04385)	910733..911698	+	966	WP_255824085.1	hypothetical protein	-
E4N75_RS04385 (E4N75_04390)	911858..912556	+	699	WP_255824087.1	hypothetical protein	-
E4N75_RS04390 (E4N75_04395)	913128..913361	+	234	WP_044979179.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
E4N75_RS04395 (E4N75_04400)	913355..913693	+	339	WP_255806270.1	endoribonuclease MazF	Toxin
E4N75_RS04400 (E4N75_04405)	913840..914148	+	309	WP_255806249.1	nucleotidyltransferase family protein	-
E4N75_RS04405 (E4N75_04410)	914132..914482	+	351	WP_255819005.1	DUF86 domain-containing protein	-
E4N75_RS04410 (E4N75_04415)	914472..915131	+	660	WP_255824089.1	hypothetical protein	-
E4N75_RS04415 (E4N75_04420)	915297..915776	+	480	WP_255824091.1	hypothetical protein	-
E4N75_RS04420 (E4N75_04425)	916151..916618	+	468	WP_255824093.1	hypothetical protein	-
E4N75_RS04425 (E4N75_04430)	916700..916945	+	246	WP_002690286.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
E4N75_RS04430 (E4N75_04435)	916945..917247	+	303	WP_255824506.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4N75_RS04435 (E4N75_04440)	917357..917593	+	237	WP_002672686.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integron	-	-	902234..921452	19218

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-110)

Antitoxin

• Antitoxin PemI-like (1-77)

Sequences

Toxin

Protein

• Length: 113 a.a.
• Molecular weight: 12866.70 Da
• Isoelectric Point: 6.2183

>T123181 WP_255806270.1 NZ_CP038803:913355-913693 [Treponema putidum]
MVNSNYVPDRGDLVWLDFTPQEGHEQKGRRPAICISQKRYNQKVGLALFCPITSHIKGYPFEIVLDNHSINGCILSDQVK
NLDYKKRSCDFIEKTTEEEINAVVDNIKLLIE

Nucleotide

• Length: 339 bp

>T123181 NZ_CP038803:913355-913693 [Treponema putidum]
ATGGTGAATTCAAATTATGTTCCCGATAGAGGCGATTTAGTTTGGCTTGATTTTACCCCGCAAGAAGGACATGAGCAGAA
AGGTCGTCGTCCTGCAATTTGCATTTCTCAAAAACGATACAATCAAAAAGTCGGACTTGCTTTATTTTGTCCTATTACAA
GCCATATAAAAGGCTATCCTTTTGAAATTGTATTGGATAATCATTCGATAAATGGCTGTATTCTTAGCGACCAAGTAAAG
AACCTTGATTATAAAAAAAGAAGTTGTGATTTTATAGAAAAAACAACAGAAGAAGAGATAAATGCTGTTGTAGATAATAT
AAAGTTGTTGATTGAATAA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8588.81 Da
• Isoelectric Point: 5.0035

>AT123181 WP_044979179.1 NZ_CP038803:913128-913361 [Treponema putidum]
MQVVVQKWGNSLGFRIPSLWAKDNNVKNGSKIEVIAEKGKIVILPQEKTLDDMLAMVTSENVHSEISTGYSVGNEEW

Nucleotide

• Length: 234 bp

>AT123181 NZ_CP038803:913128-913361 [Treponema putidum]
ATGCAGGTTGTAGTTCAGAAATGGGGTAATAGTCTCGGGTTTAGAATACCCTCTCTTTGGGCAAAAGATAATAATGTAAA
GAATGGAAGCAAAATAGAAGTAATCGCAGAAAAAGGAAAAATAGTAATTCTTCCTCAGGAAAAAACCCTTGATGATATGC
TGGCAATGGTTACTTCTGAAAATGTTCATTCGGAAATCTCTACAGGTTATTCAGTAGGTAACGAAGAATGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A562SC65